应用转录组测序高通量发掘东方粘虫 SSR 标记.PDFVIP

植物保护学报Journal of Plant Protection ，2017 ，44 （3）：377-384 DOI ：10.13802/ki.zwbhxb.2017.2016058 应用转录组测序高通量发掘东方粘虫SSR 标记 李 微 张 蕾 程云霞 罗礼智 江幸福* （中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193） 摘要：为高通量发掘粘虫Mythimna separata （Walker ）的SSR 标记，利用MISA 软件对第2 代转录组 测序获得的108 494 条粘虫unigenes 进行SSR 检测。结果显示，共发现12 483 个SSR 位点，出现频 率为11.505%，分布于10 685 条unigenes 中，发生频率为9.848%，平均每6 126 bp 含有1 个SSR 位 点。单核苷酸和三核苷酸是主要重复类型，分别占SSR 总数的75.206%和15.325%；六核苷酸重复 所占比例最小，仅为0.008%。粘虫转录组SSR 中共发现42 种重复基元，单核苷酸重复基元A/T 出 现频率最高，占总SSR 的74.141%；其次是AC/GT 和ATC/ATG ，分别占4.390%和4.094%。10 次重 复的SSR 数量最多，有6 557 个，占总SSR 的52.527%；11 次重复的为1 872 个，占14.996%；12 次以 上重复所占比例均不足5.000%。表明粘虫转录组中SSR 位点数量多、出现频率高、基元类型丰富。 关键词：粘虫；SSR；转录组；重复基元 High-throughput discovery of microsatellite markers based on transcriptome sequencing in the oriental armyworm, Mythimna separata (Walker) Li Wei Zhang Lei Cheng Yunxia Luo Lizhi Jiang Xingfu* （State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China） Abstract: In order to discover high-throughput single sequence repeat (SSR) markers of the oriental armyworm, Mythimna separata (Walker), the software MISA was used to filter 108 494 unigenes ob- tained from the next-generation transcriptome sequencing of M. separata for SSR. The results showed that 12 483 SSR loci were obtained, accounting for 11.505%of all unigenes, which were distributed in 10 685 unigenes. The frequency of occurrences for SSRs was 9.848%. On average, there was one SSR locus in every 6 126 bp. Mononucleotide and trinucleotide repeats were the main types, accounting for 75.206%and 15.325%of all SSRs, respectively. Hexanucieotide repeats were the least, which was only 0.0