毁灭柱孢抗生物质产生的摇瓶培养条件研究.pdfVIP

仲恺农业技术学院学报，21(1)：1O～13，2008 Jouma／ofZhongkai University ofAgriculture and Technology 文章编号：1006—0774(2008)01—0010—04 毁灭柱孢抗生物质产生的摇瓶培养条件研究 秦 刚 ，曾大兴 ，姜子德 (1．华南农业大学生物防治教育部工程研究中心，广东广州510642；2．深圳职业技术学院 应用化学与生物技术学院，广东深圳518055；3．成都农业科技职业学院，四川成都611130) 摘要：通过液体振荡培养，就不同培养基及初始pH、培养时间和培养温度等培养条件对毁灭柱孢 (Cytindro． carpon destructans)D-5菌株产生抗生物质的影响进行了研究．结果表明，马铃薯蔗糖培养基是D-5菌株产生抗生 物质最好的培养基；培养基初始pH 6、培养温度22 qC、摇床转速250 r／min、250 mL三角瓶摇瓶装量80 mL为 D-5菌株的最适振荡培养条件；抗生物质的产生随培养时间的延长而增加，4 d后达到最高． 关键词：毁灭柱孢 (Cylindrocarpon destructans)；抗生物质；生物防治 中图分类号：Q93—335；$476 ．8 文献标识码：A Submerged culture conditions for antifungal substances production by Cylindrocarpon destructans QIN Gang ， ，ZENG Da—xing ，JIANG Zi—de (1．Engineering Research Center of Biological Control，Ministry of Education，South China Agricultural Univemity， Guangzhou 5 10642，China；2．School of Applied Chemistry and Biological Technology，Shenzhen Polytechnic，Shenzhen 518055，Chi【Ia：3．Chengdu Vocational Couege of Agricultural Science and Technology，Chengdu 611130，China) Abstract：The effects of various media and culture conditions，including initial pH，fermentation dura— tion，culture temperature，on the production of antifungal substances by Cylindrocarpon destructans were investigated in shake—flask cultures．The results showed that potato sucrose broth was the best medium for the production of antifungal substances，and that pH 6，22 oC，250 r／min and 80 mL medium in a 200 mL—flask were found to be the optimal conditions for submerged culture．The production of antifungal substances was higher with longer culture time，and peaked after 4 days of cultivation． Key words：Cylindrocarpon destructans；antifungal substances；biocontrol 生物防治因与生态环境保护间的 “相容性”及与农业可持续发展间的 “统一性”而倍受世界各国的 重视，并在促进农林业生产发展和保护生态环境方面发挥了日益重要的作用 _3J．毁灭柱孢 (Cylind