枸杞子质量控制成分遴选研究 - 国际中医中药杂志.pdfVIP

·726 · 国际中医中药杂志2014年8月第36卷第8期 Int J Trad Chin Med, August 2014, Vol. 36, No.8 ·论著· 枸杞子质量控制成分遴选研究 潘艳丽 唐明敏 刘洋 项佳媚 王晶娟 朱美玲 隗丽 杨文宁 【摘要】 目的 观察枸杞子化学成分体内吸收代谢情况，结合肠吸收遴选枸杞子质量控制成分。 方法 采用翻转肠囊与腹主动脉采血等方法，结合HPLC 指纹图谱，探究枸杞子化学成分的肠吸收及入 血情况。结果 通过翻转肠囊实验，确定枸杞子中12 种化学成分可被小肠吸收，其中成分2 、3、4 、5、 6、9、10、11 以原型被吸收；成分 1、7、8、12 为经肠壁代谢产生的新成分被吸收。对血中多成分指纹 图谱进行分析，确定了4 种化学成分3、7、10、13 可被吸收入血，其中成分3、10 以原型直接入血，成 分7 为经代谢后吸收入血的新成分。结论 综合枸杞子肠吸收实验与入血成分分析，可初步遴选出成分3、 10、13 作为枸杞子的质量控制成分。 【关键词】 枸杞子；翻转肠囊法；肠吸收；质控成分 Selection of the quality control ingredients based on absorption of multicomponent in * Fructus Lycii Pan Yanli , Tang Mingmin, Liu Yang, Xiang Jiamei, Wang Jingjuan, Zhu Meiling, Wei Li, * Yang Wenning. Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China Corresponding author：Liu Yang, Beij ing University of Chinese Medicine, Beijing 100102, China; Email ： liuyang@bucm.edu.cn 【Abstract 】 Objective To select the components for quality control of Fructus Lycii based on the absorption of its extract. Methods To investigate metabolism of components of Fructus Lycii, everted rat gut sacs was carried out as well as the blood was taken from abdominal aorta,.and all samples were analysised by HPLC. Results There are twelve constituents absorbed between ileum and jejunum of rat ，and four constituents were detected in the blood. Compound 2, 3, 4, 5, 6, 9, 10, 11 were absorbed in prototype forms in the intestine directly ，and compound 1, 7, 8, 12 were new ones. On the other hand, four compositions （3, 7, 10, 13）could be absorbed into blood through analysis serum samples obtaining from aorta abdominalis of rats. Two of the