匹鲁卡品致大鼠癫痫模型及苔藓纤维出芽的观察.docVIP

匹鲁卡品致大鼠癫痫模型及海马苔藓纤维出芽的观察 陈丽丽，黄靓妹，詹红艳，曹亦宾 唐山工人医院 神经内科，急诊内科，河北唐山 063000 摘要：目的 观察腹腔注射匹鲁卡品后大鼠痫性发作及海马苔藓出芽。方法 Wistra大鼠60只随机分为癫痫模型组和对照组，均进行Nissl染色及Timm银染。并于实验当天行脑电图检查。结果 腹腔注射匹鲁卡品后大鼠可见急性癫痫发作，观察慢性期自发性发作。Nissl染色示模型组各区评分两两比较，结果示CA3、门区和CA1区神经元缺失差异有显著性（P＜0.05）。Timm银染示模型组与对照组苔藓纤维出芽Timm银染颗粒评分比较，结果示二者差异有显著性,P＜0.05，前者明显高于后者。结论 腹腔注射匹鲁卡品简单复制了人类癫痫的基本病理特征，是研究癫痫的一种简便方法。 关键词：匹鲁卡品；苔藓纤维；出芽；癫痫 The observation of mossy fiber sprouting in hippocampus of rat and rat epilepsy model induced by pilocarpine Chen Lili Huang Liangmei Zhan Hongyan Cao Yibin ( Department of Neurology Tangshan worker hospital HeBei TangShan 063000) Abstrac : Objective:To investigate the seizures of rats after pilocarpine injection and mossy fiber sprouting in hippocampus. Methods: 60 rats wered divided intuo two groups: epilepsy and control group.We investigated the neuronal damage, mossy fiber sprouting by Nissl and neo-Timm staining. Results: Of the animals injected with pilocarpine, 93%develped epilepsy, and most showed recurrent seizures during the chroic period. Comparention of the neuronal damage in the hilus of dentate gynus and CA3 and CA1subfied was significant in Nissl staining. Compared with control group, mossy fiber sprouting in epilepsy group was significant in Timm staining. Conclusion: The epilepsy model induced by pilocarpine in rats can replicate several the features of human temporal epilepsy. It may be a useful model for studying epilepsy. Key words: Pilocarpine; Mossy fiber; Sprouting; Epilepsy 实验动物模型是研究癫痫的重要手段。许多研究发现，腹腔注射匹鲁卡品（pilocarpine,PILO）是诱导癫痫发作的一种简单方便的动物模型 [1-2]。PILO通过同时激动乙酰胆碱和谷氨酸受体导致大鼠癫痫发作。发作后可引起分为三个阶段的行为和脑电图变化：a 急性期：边缘性癫痫持续状态（Status Epilepsy，SE）可持续至24h；b 静止期：EEG和行为基本正常，约4～42天；c：慢性自发性复发性发作（Spontaneous Recurrent Seizures，SRS），类似于复杂部分性发作的病人，每只动物约发作2～3次/周[3]。长期发作可引起类似人类颞叶癫痫的病理改变，如神经元缺失、胶质细胞增生、苔藓纤维出芽等[4-5]。因此，该模型已作为理想的动物模型之一，用于癫痫的实验研究。 1.材料与方法： 1.1.实验动物 健康雄性成年Wistra大鼠60只，体重200-250g，由华北煤炭医学院动物实验部提供。随机分为二组①癫痫模型组共30只②生理盐水对照组共30只。均进行Nissl染色及Timm银染。 1.2.药品 匹鲁卡品（PILO）美国SIGMA公司，