神经节内板状末梢是豚鼠食道迷走传入神经末梢的机械敏感性受体.pdfVIP

Acta Physiologica Sinica , April 25, 2006, 58 (2): 171-176 171 Research Paper Intraganglionic laminar endings act as mechanoreceptors of vagal afferent nerve in guinea pig esophagus * YANG Xia , LIU Ran Qilu Hospital, Shandong University, Jinan 250012, China Abstract: Intraganglionic laminar endings (IGLEs) have been supposed to be the mechanoreceptors in the gut by electrophysiological recording techniques. But the specialized morphology of IGLEs could not be displayed closely associated with this function and the mechanism that IGLEs act as the mechanotransduction sites in the gut is not yet well understood. In the present study, we used styryl dye FM1-43 combined with stretch stimulation in the guinea pig esophagus to test whether IGLEs acted as the mechano-sensitive receptors of the vagal afferent nerves. At the same time, the special structure of IGLEs displayed by FM1-43 was further confirmed by neurobiotin anterograde labeling technique. To further investigate the characteristics of IGLEs as mechanosensitive receptors, different drugs were used to block or stimulate IGLEs activation. Our results indicated that only in the stretched preparation could FM1-43 enter the IGLEs and completely display their specialized structure, which was consistent with that shown by neurobiotin. The amount of IGLEs shown by stretch-evoked FM1-43 staining was much more than that shown without stretch stimulation [(90.4±9.5)% vs (10.7±2.1)%, P0.05]. Ca2+, TTX (0.6 µmol/L), atropine (0.6 µmol/L), SKF (50 µmol/L), and gadolium (100 µmol/L) had no effect on the IGLEs activation. But for benzamil (100 µmol/L), an epithelial sodium channel blocker, activation of IGLEs by stretch stimulation was significantly blocked. The potent ATP analogue, α,β-methylene ATP (100 µmol/L) could not activate FM1-43 staining without stretch. These results indicate that IGLEs are