MZY-02盾叶秋海棠药材镇痛及抗凝血作用的初步实验研究.docVIP

盾叶秋海棠药材镇痛及抗凝血作用的初步实验研究 摘要：目的观察盾叶秋海棠根茎乙醇提取物（ALE）和水提取物（AQE）的镇痛、抗凝血作用及其急性毒性。方法采用小鼠扭体法、热板法断尾出血时间(BT)及最大耐受量（MTD）测定法。结果不同剂量的ALE能明显减少醋酸致小鼠扭体次数及延长热板致痛的潜伏期ALE大剂量时能明显延长小鼠BTALE和AQE小鼠口服MTD为3.865gkg-1和7.175gkg-1 (相当于干燥原生药50gkg-1) ，间隔12h，共3次。 结论盾叶秋海棠根茎的ALE具有显著的镇痛及抗凝血作用，且盾叶秋海棠根茎的ALE和AQE的急性毒性均较小。 关键词：盾叶秋海棠药材；镇痛；抗凝血；急性毒性 Study on the Analgesic and Arresting Bleeding Effects of Begonia cavalerei Abstract: Objective To observe the analgesic and anticoagulative effects of alcoholic extract (ALE)and the aqueous extract (AQE) of rhizome of Begonia cavalerei herb from Guizhou， and to determine the acute toxicity of the extracts. Methods The indexes were tested as follows: writhes of abdominal cavity on mice caused by ip of acetic acid， the incubation of pain induced by heated plate stimulation in mice ， bleeding time (BT) by cutting the mouse，s tail and the maximal tolerance dose (MTD). Results ALE could obviously decrease the numbers of writhe and prolong the incubation of pain of mice. ALE could obviously shorten BT of mice. MTD of AQE for mice was 7.175g?kg-1 and that of ALE was 3.865g?kg-1 by ig three times in 12h (be equal to 3×50g?kg-1 of dried herbs in 12h). Conclusion The pharmacological basis of ALE of rhizome of Begonia cavalerei herb on analgesia and anticoagulation are proved. The acute toxicity of ALE and AQE of rhizome of Begonia cavalerei herb is slight. Key words: Begonia cavalerei herb；analgesia；anticoagulation；acute toxicity 秋海棠科植物盾叶秋海棠Begonia cavalerei Lévl.以新鲜或干燥根茎入药，具有散瘀止痛等功效，用于痛经，闭经，跌扑肿痛等，是贵州省少数民族常用药[1]。根据其功效主治对黔产盾叶秋海棠干燥根茎的乙醇提取物（ALE）和水提取物（AQE）的镇痛、抗凝血作用及其急性毒性进行了初步实验研究，现报道如下。1 仪器与材料1.1 仪器 AG135型电子天平（瑞士Mettler-Toledo公司）；GJ-8402型(55.0±0.2℃)热板测痛仪（浙江宁海白石电子医药仪器厂）。 1.2 动物 小白鼠，昆明种，清洁级。由贵阳医学院实验动物中心提供，合格证号：SCXK（黔）2002-0001。 1.3 供试药材采自贵阳市鬼架桥，经贵阳中医学院何顺志教授鉴定为盾叶秋海棠B cavalerei Lévl.的根茎，晒干。 ①乙醇提取物（ALE）将供试药材粉碎成粗粉，50℃干燥，取1500g，置圆底烧瓶中，用无水乙醇（为药材量的4倍）回流提取4次，每次4h，过滤，合并滤液，减压回收溶剂至近干，再经60℃真空干燥后得ALE干浸膏（得膏率为7.73 %）。临用时配制成一定浓度的含0.4%Tween-80的水混悬液。②水提取物（AQE）将乙醇提取后的药渣在室温下挥干溶剂至无乙醇味，取13