HPLC测定复方维生素注射液中维生素A、E、K1含量.docVIP

HPLC测定复方维生素注射液中维生素A、E、K1含量【摘要】 目的 探讨高效液相色谱法(high performance liquid chromatography,HPLC)测定复方维生素注射液A、E、K1含量的可行性。方法 采用HPLC法，C18色谱柱(150 mm×46 mm,5 μm)，流动相：甲醇-酒精(80∶20)，检测波长270 nm。结果 维生素A棕榈酸酯在125~125．5 μg/ml之间线性良好(r=0．999 9),平均回收率为99．38%，RSD为1．36%； 维生素E在125~125．5 μg/ml之间线性良好（r=0．999 9）,平均回收率为95．87%，RSD为0．95%。维生素K1在16．5~166．5 μg/m之间线性良好(r=0．999 8)，平均回收率为98．08%，RSD为1．57%。结论 HPLC法测定复方维生素注射液维生素A、E、K1的含量专属性强，灵敏度高，准确性好。 【关键词】高效液相色谱法；维生素A；维生素E；维生素K1 Determination content ofcompound vitamins A、E、K1 injection by high performance liquid chromatography XI Ying.Department of Pharmacy,Affiliated Hospital of BengbuMedicalCollege,Bengbu ，Anhui233004，China 【Abstract】 Objective To determine the contents in compound vitamins A、E、K1 injection by high performance liquid chromatography(HPLC).Methods HPLC was used C18column(150 mm×46 mm,5 μm)was selected,the mobile phase was methanol-alcohol(80∶20).Detection was performed by UV with wavelength of 270 nm.Results A linearity of retinyl palmitate was obtained from 125 to 125．5 μg/ml with good correlation(r=0．999 9).The average recovery of retinyl palmitate was 99．38%,and RSD Was 1．36%.A linearity of vitamin E was obtained from 125 to 1251 μg/mlwith good correlation(r=0．999 8).The average recovery of vitamin E was 98．87%,and RSD was 0．95%.A linearity of vitamin K1 was obtained from 16．5 to 166．5 μg/ml with good correlation(r=0．999).The average recovery of vitamin K1 was 98．08%,and RSD was 1．57%.Conclusion The RP-HPLC is sensitive and accurate in determining thecontents in compound vitamin injection. 【Key words】Highperformance liquid chromatography；Vitamin A；Vitamin E；Vitamin K1 维生素(vitamin)是维持人体正常物质代谢和某些特殊生理功能不可缺少的低分子有机化合物，主要参与各种酶的组成，因其结构和理化性质不同，使其各具特殊的生理功能。复方维生素注射液可作为全营养静脉输液的添加剂,以满足每日的维生素需要[1]。近来有系统测定维生素的报道，但多数是报道纯品维生素的分离分析。本文应用高效液相色谱法(high performance liquid chromatography,HPLC)测定复方维生素注射液A、E、K1含量，现作报告。 1 仪器与试剂 仪器：岛津LC-10A高效液相色谱仪系统，包括LC-10ATvp泵，SPD-10Avp紫外检测器，C-R8A色谱数据处理仪。色谱柱：以十八烷基硅烷键合硅胶为填充剂(150 mm×46 mm，5 μm),不锈钢柱(Sh