人生长激素对人胃-结肠癌细胞株细胞周期影响.docVIP

人生长激素对人胃\结肠癌细胞株细胞周期影响【摘要】 目的 了解重组人生长激素(rhGH)在体外对人胃、结肠癌细胞有无促进增殖作用。方法 实验分对照组、rhGH组、奥沙利铂(L-OHP)组和L-OHP+rhGH组,利用体外细胞培养及流式细胞仪等方法,测定不同浓度的rhGH对人胃癌细胞株MKN45、人结肠癌细胞株Colo320细胞周期和增殖指数(PI)的影响。结果 rhGH在体外不促进MKN45和Colo320细胞分裂增殖。rhGH组与对照组、L-OHP+rhGH组与L-OHP组比较均无统计学意义(P0.05),L-OHP+rhGH组与对照组比较或L-OHP+rhGH组与对应的rhGH组比较,阻滞于G0~G1期的细胞数增加,S期和G2~M期细胞明显减少,PI明显降低(P0.05).The cells arrested in G0~G1 phase obviously increased,meanwhile,the cells in S phase and G2~M phase and PI distinctly decreased compared L-OHP+rhGH group with control group or between L-OHP+rhGH group and rhGH group in comparison(P0.05)。S期:L-OHP组和L-OHP+rhGH组比对照组和rhGH组降低(P0.05),但有升高趋势。G2~M期:L-OHP组和L-OHP+rhGH组比对照组和rhGH组降低(P0.05);同时,L-OHP+rhGH组与对应的rhGH组及对照组比较,G2~M期细胞也减少(P 表2 药物作用后MKN45和Colo320细胞株各组的增殖周期百分数( x±s) 组别 MKN45(%)Colo320(%) G0~G1SG2~MG0~G1SG2~M 对照组51.5±1.736.2±1.512.3±3.325.6±3.240.1±1.634.2±4.4 L-OHP组81.8±1.5?*8.9±0.7?*9.3±1.257.4±5.4?*25.3±2.8?*17.3±3.4?* rhGH1组54.6±3.433.5±6.511.9±3.326.4±2.539.9±1.533.6±2.7 rhGH2组53.8±4.134.7±6.111.5±1.625.9±3.741.0±2.234.0±3.4 rhGH3组52.1±4.235.8±7.312.1±3.526.9±3.142.1±2.230.9±2.0 rhGH4组53.9±4.733.9±7.812.2±3.925.4±4.241.9±2.632.7±2.0 L-OHP+rhGH1组82.5±3.4?*7.7±3.8?*9.8±1.957.3±3.0?*26.8±3.1?*15.9±1.1?* L-OHP+rhGH2组81.2±2.3?*8.6±1.9?*10.2±0.955.7±4.3?*25.8±5.5?*18.5±3.1?* L-OHP+rhGH3组83.1±1.8?*8.1±5.0?*8.8±4.556.1±7.2?*27.0±5.0?*16.8±4.5?* L-OHP+rhGH4组82.9±2.9?*7.4±1.6?*9.7±2.456.4±5.2?*26.4±3.6?*17.1±1.1?* 注:与对照组和rhGH各亚组比较,?*P0.05),见表3。 表3 药物作用后MKN45和Colo320细胞株各组 细胞增殖指数( x±s) 组别MKN45(%)Colo320(%) 对照组48.5±1.975.4±3.2 L-OHP组18.2±1.6?*42.6±5.3?* rhGH1组45.4±4.673.9±2.9 rhGH2组46.2±3.874.1±3.7 rhGH3组47.9±4.273.1±3.1 rhGH4组46.1±5.374.6±4.2 L-OHP+rhGH1组17.5±2.5?*42.7±3.0?* L-OHP+rhGH2组18.8±2.9?*44.3±4.3 L-OHP+rhGH3组16.9±1.4?*43.9±7.2?* L-OHP+rhGH4组17.1±2.5?*43.6±5.12?* 注:与对照组和rhGH各亚组比较, ?*P0.05),表明rhGH对体外培养的胃、结肠癌细胞无促进增殖的作用。在rhGH与L-OHP合用与对照组和rhGH组比较,S期及G2~M期的细胞及PI明显减少(P0.05)。为临床胃肠道肿瘤患者术后应用