膀胱癌患者CYP1A1基因MSPI多态性相关研究.docVIP

膀胱癌患者CYP1A1基因MSPI多态性相关研究【摘要】 目的 探讨细胞色素CYP1A1基因MSPI多态性与膀胱癌遗传易感性的关系。方法 应用聚合酶链式反应- 限制性片段长度多态性(PCR-RFLP)分析技术，检测44例膀胱癌患者(病例组)和85例同期住院非膀胱癌患者(对照组)，检测CYP1A1基因MSPI多态性位点的3种基因型及等位基因的分布频率。结果 在病例组CYP1A1基因MSPI位点基因型分布频率为：TT（54．5％）、TC（36．4％）、CC（9．1％），等位基因分布频率为T（72．7％）、C（27．3％）；在对照组CYP1A1基因MSPI位点基因型的分布频率为TT（61．2％）、TC（31．2％）、CC（7．1％），等位基因分布频率为T（77．1％）、C（22．9％）。各个基因型在两组中所占的比例差异无统计学意义（P＞0．05）;T、C等位基因频率两组比较差异亦无统计学意义(P＞0．05)。结论 CYP1A1基因MSPI位点多态性的单独存在可能与本地区膀胱癌易感性无关。 【关键词】 膀胱肿瘤；CYP1A1；基因多态性；易感性 Analysis of the polymorphism of CYP1A1-MSPI gene in patients with bladder cancerYANG Li-xin，ZHANG Hai-feng，CUI Wen-peng,et al．Dept of Urology，the First Hospital，Jilin University，Changchun 130021,China 【Abstract】 Objective To discuss the relationship between the polymorphism of cytochrome CYP1A1-MSPI gene and genetic predisposition of bladder cancer．Methods The distribution frequence of allelic and three genotype of CYP1A1-MSPI polymorphism site of 44 patients with bladder cancer(case group) and 85 patients without bladder cancer(control group) by polymerase chain reaction- restriction fragment length polymorphism(PCR-RFLP) were detected．Results The distribution frequences of genotype of CYP1A1-MSPI site of the case group were TT（54．5％）,TC（36．4％）,CC（9．1％）,and the frequences of allelic gene were T（72．7％）,C（27．3％）．While the distribution frequences of genotype of CYP1A1-MSPI site of the control group were TT（61．2％）,TC（31．2％）,CC（7．1％）,and the of allelic gene were T（77．1％）,C（22．9％）．The ratio of genotype in each group has no significant difference（P＞0．05）；the frequence of allelic gene of T and C in each group also had no significant difference（P＞0．05）．Conclusion There may be no relationship between the individualism of CYP1A1-MSPI site polymorphism and susceptibility of bladder cancer in this area． 【Key words】 Ovarian tumor;CYP450;Gene polymorphism 膀胱癌是泌尿系统常见恶性肿瘤之一，其发生、发展与环境因素、遗传因素及他们的相互关系等密切相关。其中个体遗传易感性是影响膀胱癌发病的一个重要因素[1]。CYP1A1是细胞色素P450(CytochromeP450，CYP)家族的一名成员，是活化二恶英等环境毒素的主要酶类[2]，而酶代谢能力的