浅析标准偏差.pptVIP

Chapter 2 Chromatography General Principles Experiment of Tswett Basic Conceptions Chromatography Mobile phase Stationary phase Principle of chromatography 2.2 Classification of Chromatographic Methods Different types of ChromatographyForm of Stationary Phase ： column C. and planar C.State of Mobile Phase：  Gas C. , Liquid C. , Supercritical fluid C. Mechanism of interaction:  Distribution C. and adsorption C. More about the column chromatographic methodsGC: GSC and GLCLC: LSC and LLC, HPLCCapillary chromatography and ECfrontal Chromatography and dispalcement Chromatography 2.3 Chromatographic Behavior Elution in column chromatography Chromatogram and Terms Important Parameters Partition coefficient (Distribution Constants)Capacity Factor (Retention Factor) Selective Factor Phase ratio Terms of Chromatogram Partition coefficient （分配系数） CS concentration in stationary phase CM concentration in mobile phaseequilibrium constant for distribution of solute between two phases Capacity Factor （分配比） Selectivity Factor Also called separation factor, is a measure of the separation of two substances Glossary Chromatogram, GC, LC, HPLC Distribution Adsorption Planar Column Immiscible Mobile phase Stationary phase Elution and eluent K, k, αβand the terms of chromatograms Classification of column chromatography according to the mobile and stationary phases used 2.4 Chromatographic theory Peak broadening, column efficiency and separated effect Plate theory and the number of theoretical Plates Rate theory and the Van Deemter Equation A Quantitative Description of Resolution Plate theory  Logical transposition of discrete partition steps in the column, and the elution plot can be expressed as: Discussion of the Plate theory Assumption and limitation The number of theoretical plate N Plate Height H Determination of N and H Van Deemter Equation H=A+B/u+C u Eddy diffusion term A（涡流扩散项） A=2λdp Longitudinal diffusio