献给初学者western blot操作步骤2012版（For beginners western blot operation step 2012）.docVIP

下载本文档

37
0
约2.42万字
约 18页
2017-08-04 发布于河南
举报
版权申诉

献给初学者western blot操作步骤2012版（For beginners western blot operation step 2012）.doc

1、本文档共18页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

献给初学者western blot操作步骤2012版（For beginners western blot operation step 2012） To beginners: western blot steps 2012 edition in 2011, I was working as a western, found that the data is very old, most of the online information is make private copies, each other different ways, and combining the current online can find information and personal practical experience, the editor of the western blot 2012 version of the process, including main operation steps and the need to pay attention to useful detail, with references (forgive me if I cant like Endnote indicate the literature origin, can probably identify the reference resources), I hope it can help you. I believe that you will be able to follow the steps that have been made to stabilize the repeatable, clean western band. In order to save time, I summarized the western blot Quick Guide and the western specific time plan, attached to the end of the text. It is hard to be wrong to make such a long post, and welcome criticism. Background: The protein imprint was invented by George Stark at Stanford university. Neal Burnette was first known as Western Blot in the AnalyticalBiochemistry of 1981. Began to do the most imprinting is a scientist called Southern, but mark the object is a strand of DNA, he put the technology known as the Southern blot, later in the two processes are similar, but different imprinting method, one for RNA and a protein, the people of the two technologies respectively referred to as the Northern and Western, has nothing to do with the two technology inventor. 1. Sample preparation of protein: As this step is different, please refer to the instructions of the kit. The sample preparation of protein is the first step in Western Blotting. Sample preparation is the key step and requires the best possible access to all proteins. Attention should be paid to the following questions: should maintain maximum solubility and repeatability of proteins under proper salt concentration. selects suitable surfactants a