第二篇动物细胞工程（Second animal cell engineering）.doc

第二篇动物细胞工程（Second animal cell engineering） Second animal cell engineering Summary The fourth chapter cell culture The fifth chapter cell fusion and monoclonal antibody The sixth chapter embryo engineering The seventh chapter of stem cell and tissue engineering The eighth chapter nuclear transplantation technique and animal cloning The ninth chapter of transgenic animal and animal bioreactor The tenth chapter animal chromosome engineering Summary The concept of an animal cell engineering The method of biology and molecular cell biology applications, genetic manipulation in animal cell level. Is a matter of changing the genetic material technical field. Cell and tissue culture cell fusion Chromosome engineering Embryo engineering (embryo transplantation, IVF) Cell recombination and cloning technology (biological replication) Transgenic animal and bioreactor Two, the main technical field of animal cell engineering involved The fourth chapter cell culture 1, cell culture development history and relevant concepts In 1907, American biologist Harrison of the frog embryo nerve tissue culture in lymph, keep alive for a few weeks, and observe the growth of neurite. Known as the father of the cell culture (organization). The aseptic technique of Carrel1912, 1923 designed the cards culture bottle. Now, the technology of cell culture has become an important means in modern medical research. In recent years, cell culture techniques have been widely used in cell biology, molecular biology, genetics, pharmacology, immunology, virology, oncology, gerontology and clinical disciplines, and achieved fruitful results. 1) cell culture (cell culture) Refers to the tissue removed, with chemical or physical methods, be dispersed into single cells were cultivated in vitro. No cell tissue formed in the process of training. Culture is a single cell or group of cells. Broadly speaking, in vitro culture (in vitro) including all levels of culture structure, namely culture organ culture, tissue cul