久效磷对细小色矛线虫胚胎发育和繁殖的影响.PDF

中国环境科学 2009,29(5) ：543~547 China Environmental Science 久效磷对细小色矛线虫胚胎发育和繁殖的影响 * 王 摆,汝少国 ,王 蔚 ( 中国海洋大学海洋生命学院, 山东 青岛 266003) 摘要：采用平板静态毒性实验方法,研究了久效磷对细小色矛线虫(Chromadorina germanica) 的急性毒性及胚胎发育和繁殖的影响,并探讨 了其毒性作用机制.结果表明,久效磷对 C. germanica 的 24,48,96h 的 LC50 分别为 66.42,36.94,16.03 µmol/L.对 C. germanica 繁殖具有显著 的抑制作用:1.0µmol/L 久效磷暴露组子一代的数量急剧减少,仅为对照组的 52.9%,10µmol/L 暴露组则完全抑制了产卵和子一代的产生.对 C.germanica 的胚胎毒性主要表现为胚胎发育时间的延迟:0.1,1.0,10.0µmol/L 久效磷暴露组胚胎发育持续时间比对照组的 17.05h 分别延长 了 1.24,1.92,1.96h,呈剂量-效应关系,且 10.0µmol/L 久效磷完全抑制了卵的孵化.久效磷对 C. germanica 精巢和卵巢超微结构的损伤,导致了 精子和卵子质量下降.因此,久效磷导致 C.germanica 的胚胎发育持续时间的延长和精子、卵子质量下降是其子一代数量急剧减少的主要原 因之一. 关键词：细小色矛线虫；久效磷；胚胎发育；繁殖；超微结构 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2009)05-0543-05 Effect of monocrotophos on embryonic development and reproduction of a marine nematode Chromadorina * germanica . WANG Bai, RU Shao-guo , WANG Wei (College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China). China Environmental Science, 2009,29(5)：543~547 Abstract ：Acute toxicity of monocrotophos on Chromadorina germanica was tested in static culture plate, and effects of monocrotophos on embryonic development and reproduction were also studied. LC50 of the 24, 48 and 96 h were 66.42, 36.94, 16.03 µmol/L,respectively. Monocrotophos significantly inhibited the reproduction of C. germanica. When exposed to 1.0 µmol/L monocrotophos, the number of offspring reduced to 52.9% compared with control, while exposed to 10 µmol/L monocrotophos, neither eggs nor offspring were observed. The sharp reduction of the number of offspring might relate to the interruption of embryo development and damage on testis and ovary. After exposure to 0.1, 1.0, 10.0 µmol/L monocrotophos, the development of embryo was