响应面法优化油橄榄叶中橄榄苦苷超声辅助提取工艺.PDF

※工艺技术 食品科学 2012, Vol. 33, No. 18 73 闫树军，王 远，苏艳红，饶在生，李 晖 * ( 四川大学化学工程学院，四川 成都 610065) 目的：研究油橄榄叶中橄榄苦苷超声辅助提取最佳工艺条件。方法：选定甲醇体积分数、料液比和提 取时间作为影响因素，以橄榄苦苷提取率为评价指标。在单因素试验的基础上，通过 3 因素3 水平 Box-Behnken 试 验，建立橄榄苦苷提取率的二次多项式回归方程，经响应面回归分析得到优化组合条件。结果：最佳工艺条件为 甲醇体积分数93.5% 、料液比 1:25(g/mL)、提取时间26min 。结论：油橄榄叶中橄榄苦苷提取率可达7.83% 。 油橄榄叶；橄榄苦苷；超声提取；响应面分析 Optimization of Ultrasound-Assisted Extraction Process for Oleuropein from Olea europaea Leaves by Response Surface Analysis YAN Shu-jun ，WANG Yuan ，SU Yan-hong，RAO Zai-sheng ，LI Hui* (College of Chemical Engineering, Sichuan University, Chengdu 610065, China) Abstract ：Objective: To find optimal process conditions for the ultrasound-assisted extraction of oleuropein from Olea europaea leaves. Methods: The effects of methanol concentration, solid-to-solvent ratio and extraction time on oleuropein yield were investigated by one-factor-at-a-time design. A three-factor, three-level Box-Behnken experimental design was employed to build a second-order polynomial regression equation. The obtained regression equation was analyzed by response surface analysis to optimize the three process conditions. Results: The optimal process conditions were as follows: methanol concen- tration of 93.5%, solid-to-solvent ratio of 1:25 (g/mL) and an extraction time of 26 min. Conclusion: Under these conditions, the oleuropein yield from Olea europaea leaves was up to 7.83%. Key words ：Olea europaea leaves ；oleuropein ；ultrasound-assisted extraction ；response surface methodology 中图分类号：TS201.1 文献标识码：A 文章编号：1002-6630(2012)18-0073-04 油橄榄(Olea europare Linn) 又名洋橄榄、齐墩果、 是利用超声波的空化效应增大介质分