高聚物萃取-光度法测定水果硬糖中色素的含量-食品科学.PDF

※分析检测 食品科学 2012, Vol. 33, No. 18 191 - 杜建中，董柳余，金 蓓 (湛江师范学院化学科学与技术学院，广东 湛江 524048) 　　　　　　　 利用水溶性高聚物溶液在无机盐存在条件下可分成两相的特点，将水果硬糖中的色素转入高聚物相，采 用单波长法和双波长等吸收法对色素含量进行定量测定。结果显示，胭脂红在 0.2～30 μg/mL 、柠檬黄在0.2～40 μg/mL 、 2 诱惑红在 0.2～35 μg/mL、日落黄在0.2～30 μg/mL、亮蓝在0.1～10μg/mL 范围内有良好的线性关系，确定系数(R ) 均大于 0.997 。该方法所需仪器设备简单，操作简单，准确度较高。 聚乙二醇 -4000 ；水果硬糖；分光光度法；色素 Determination of Pigments in Fruit Hard Candy by Polymer Extraction and Spectrophotometry DU Jian-zhong ，DONG Liu-yu ，JIN Bei (School of Chemistry Science and Technology, Zhanjiang Normal University, Zhanjiang 524048, China) Abstract ：Based on the fact that aqueous solution of water-soluble polymers can be separated into two phases upon addition of inorganic salt, pigments in fruit hard candy were transferred into the polymer phase, resulting in reduced interference, and quantitatively determined by single-wavelength or dual-wavelength spectrometry. The results showed that the linear ranges of cochineal, lemon yellow, fancy red, sunset yellow, and bright blue were 0.2 －30, 0.2 －40, 0.2 －35, 0.2 －30 μg/mL and 0.1 －10 μg/mL, respectively, with correlation coefficient (R2) of larger than 0.997. This method needs only simple equipment and is easy to use, and accurate. Key words ：polythene glycol-4000 ；fruit hard candy ；spectrometry ；pigment 中图分类号：O658.2 文献标识码：A 文章编号：1002-6630(2012)18-0191-04 常见的食用合成色素主要有胭脂红、苋菜红、日 的色素定量转移至聚乙二醇-4000 相中，利用单波长或 落黄、柠檬黄、新红、诱惑红、亮蓝等，它们进入 者双波长等吸收法，实现糖果中不同色素含量的定量 人体后不仅无营养价值，而且过量摄入其化学构成物质 测 定 。 本身对人体有害，会消耗体内解毒物质，干扰人体正