骨组织力学敏感性的调控龚啸元潘君重庆大学生物工程学院重庆.DOC

骨组织力学敏感性的调控 龚啸元，潘君 （重庆大学 生物工程学院，重庆 400044） 摘要 目的：力学刺激能够通过增加骨生成作用，抑制骨消融作用促使骨密度的增加。然而骨组织的力学敏感性在长期的力学加载过程中呈逐渐下降的趋势，其调控机理尚不明确。 方法：通过总结既往文献并结合本课题组前期研究结果，分析成骨细胞细胞骨架对其力学敏感性的调控作用。 结果：成骨细胞中细胞骨架的微丝结构与细胞的力学响应密切相关。胞内钙离子信号与细胞骨架的交互作用在成骨细胞力学敏感性的调控中起主导作用。 结论：在流体剪应力加载模型中，持续不变的力学刺激使得细胞通过增强细胞骨、细胞硬度来适应其所处的力学环境；细胞也因此不再容易产生由同等水平力学刺激导致的形变，导致骨细胞的骨生成作用维持现有的水平。然而，成骨细胞中的其他力学感应装置是否同样参与了成骨细胞力学敏感性的调控还不十分清楚。解答这些疑问能够更好地为探讨骨组织以及细胞水平的力学响应机理，甚至为某些骨合成相关疾病的治疗提供重要的理论依据。 关键词：力学刺激；骨组织；力学敏感性；细胞骨架 ________________ 作者简介：龚啸元、男、博士研究生、生物力学、发表论文数量：2、联系方式（电话E-mail: sliegxy@） [基金项目]国家自然科学基金资助项目 . 通讯作者：潘君、联系电话E-mail: panj@ Regulation of mechanosensitivity in bone tissue Abstract Objective: Mechanical loading is able to increase bone mass though regulating the balance between bone formation and resorption. However, the mechanosensitivity of bone gradually decreases during continuous mechanical loading. The underlying mechanism still remains unknown. Methods: By combining results form previous studies and our present studies, we set out to explain the role of cytoskeleton in mechanosensitivity regulation in osteoblasts. Results: F-actin stress fiber is closely related to mechanical loading induced cellular responses. The interaction between intracellular calcium response and F-actin remolding plays a central role in the regulation of osteoblasts’ mechanosensitivity. Conclusion: When osteoblasts are stimulated by fluid shear stress, the constant force act on the cell membrane prompt the cellular adaptation to the mechanical environment by means of prompting F-actin polymerization and increasing cell stiffness. Cells are no longer response to outer force with cellular deformation. The bone formation thus ceases. However, whether other mechanosensors in osteoblasts also contribute to this regulation process are still unclear. To address these questions would be great helpful to uncover the mechanism in bony response to mechanical loading, and the development