二氧化硫暴露对拟南芥叶片形态和生理生化指标的影响.PDFVIP

业环境科学学报 园园8熏 7穴2雪:525-529 允燥怎则灶葬造 燥枣 粤早则燥鄄耘灶增蚤则燥灶皂藻灶贼 杂糟蚤藻灶糟藻 二氧化硫暴露对拟南芥叶片形态和生理生化指标的影响 李利红袁仪慧兰袁王 磊袁李秀芬 渊山西大学生命科学与技术学院袁 山西 太原 030006 冤 要院采用室内培养及密闭箱静态熏气方法，研究了不同浓度SO2 暴露对拟南芥（A rabidop s is thaliana ）叶片形态和生理生化指 标的影响。结果显示，拟南芥暴露于SO2 14 d 后，叶面气孔开度变小。浓度10 mg ·m-3 组 进植株生长发育，SOD 和CAT 活性无明显 改变，30 mg ·m-3 SO 组3 种酶活性呈诱导性增高，其中POD 增幅最大，SO 浓度90 mg ·m-3 时，CAT 活性被抑制，丙二醛（MDA ）含 2 2 量增加，株高、叶片数和叶片面积显著降低，可溶性蛋白含量减少，叶片出现明显可见伤害。研究结果表明，在低浓度条 下，拟南芥 够通过调节气孔开放、细胞抗氧化酶活性等使植株适应SO2 胁迫，但是高浓度SO2 暴露会引起细胞氧化损伤，影响植物生长发 育。 ； ；叶片形态；抗氧化酶 关键词院拟南芥 SO ；生长发育 2 中图分类号院X503.233 文献标识码院A 文章编号院1672-2043(2008)02-0525-05 Effects of Sulfur Dioxide on the Morphological and Physiological Biochemical Parameters in Arabidopsis Thaliana Plants LI Li-hong, YI Hui-lan, WANG Lei, LI Xiu-fen (School of Life Science and Technology, Shanxi University, Taiyuan 030006, China) Abstract: Effects of SO exposure on the morphological and physiological biochemical parameters were investigated in model plant A rabidop 2 s is thaliana. Test plants were exposed to SO2 at concentrations of 10, 30 and 90 mg 窑m-3 , respectively, 4 h per day for 14 d in an environment- controlled chamber, and then some parameters were measured by detecting the stomatal aperture, activities of antioxidant enzymes and growth rate. The results showed that stomatal aperture and the ratio of stomatal opening area decreased with an increase of the amount of closed stomata when plants exposed to SO . The activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) increased dose-de原 2 pendently in A rabidop s is thaliana plants. 10 mg 窑m-3 SO2 exposure had no markedly effect on the activities of SOD and CAT, and showed a positive