IGF-1对大鼠局灶性脑损伤Bcl-2表达影响.doc

IGF-1对大鼠局灶性脑损伤Bcl-2表达影响摘要： 目的：研究胰岛素样生长因子-1（IGF-1）对大鼠局灶性脑缺血再灌注后Bcl-2 表达 的影响及与缺血时间关系，探讨IGF-1对脑缺血再灌注损伤的保护作用。方法：制作SD大鼠 大脑中动脉缺血再灌注模型。将55只SD雄性大鼠随机分为假手术组（n=5）、对照组（ n=25 ）、IGF-1治疗组（n=25），其中后两组按缺血再灌时间(6h，12h，1d，3d，7d)不同 可分为 5个亚组，每组5只，治疗组于缺血2h再灌注1h后经腹腔注入40μg/kg稀释为1ml 的IG F-1，假手术组及对照组同时腹腔注入生理盐水1ml。以上动物均在再灌注后规定时间点用4 % 多聚甲醛经心脏灌注固定，取大鼠脑组织，应用免疫组化S-P法和HE染色检测Bcl-2蛋白表 达 及脑组织结构病理变化。结果：与对照组相比，治疗组大鼠脑组织Bcl-2表达明显增加，其 神经细胞坏死程度明显减轻。结论：IGF-1在大鼠局灶性脑缺血再灌注损伤中起保护作用， 其作用机制包括增加Bcl-2蛋白的表达，发挥其神经保护作用。 关键词：胰岛素样生长因子-1；局灶性脑缺血；Bcl-2；再灌注损伤 中图分类号： Q959.837; R743 文献标识码： A 文章编号： 1008-2409(2008)05-0891-03 Effects of IGF-1 on expression of Bcl-2 in rats following focal cerebral injur e/LI Kai-rong，LI Wang-hui，XIAO Hong-wen，et al.∥Department o f Anatomy， Luzhou Medical College， Luzhou 646000, China Abstract: Objective: To evaluate the protective effect of insulin-like growt hfactor-1(IGF-1) on focal cerebral ischemia reperfusion in rat. Methods: Theh ealthy adult of male SD rats(55) were divided randomly into sham operation group (5),model group(25) and IGF-1 group(25), each of the model group and IGF-1 gr ou p was further divided into 5 subgroups according to the duration of reperfusion( 6,12,24hours,3 day,7 day) after two hour of cerebral ischemia. The n the rats in sham operation and model group were injected normal saline. IGF -1 group were injected IGF-1 through abdominal cavity. Brain tissue of rats we reused to observe nerve cell injury under light microscope after HE staining, andpositive neurons of Bcl-2 were observed by immunohistochemical method. Results : Compared with the model group, the injury degree of neuron greatly reduced i nthe IGF-1 group(P0.05). At the same time, the mumber of Bcl-2 positive ne uron w as increased significantly in IGF-1 group compared with model group (P0.05 ). Co nclusion: IGF-1 plays a protective role in rat focal cerebral ischemia-reperfu si on injury, the nervous protective mechanism of IGF-1 may involve in increasingBcl-2 activity.? Key words: insulin-like