原花青素对狗离体冠状血管影响在药理学范畴探究.doc

原花青素对狗离体冠状血管影响在药理学范畴探究【摘要】目的：研究原花青素在狗冠状动脉上舒张特征和机制。方法：制备狗冠状动脉血管环，固定于盛有K-H液的恒温肌槽内，并观察等长时血管张力的变化。结果: 原花青素使KCl及无钙中CaCl2量效曲线明显右移，使肌条敏感性和最大收缩反应明显降低。原花青素可使KCl预收缩冠状动脉血管环产生明显的舒张作用，Nω-L-硝基精氨酸、甲烯兰及去内皮细胞组，可部分阻断17β-雌二醇的舒张作用。结论：原花青素具有内皮依赖性和非内皮依赖性的舒血管效应，前者的作用机制与NO和NO-cGMP途径相关，而后者可能是由于原花青素对血管的直接作用引起，即抑制电压依赖性的钙通道。 【关键词】原花青素；冠状血管；血管张力；内皮细胞；电压依赖性钙通道 文章编号：1009-5519（2007）16-2373-03 中图分类号：R9 文献标识码：A Pharmacological profile of procyanidins on canine coronaries in vitro studies HUANG Yu-lan，LIU Jing （Department of Chemistry and Lifescience，Xiangnan University， Chenzhou 423000，China） 【Abstract】Objective：To investigate the relaxative characteristics of procyanidins on Coronary arteries in the dog and its mechanism. Methods：Rings of Canine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution， and then isometric tension was measured.Results：PCs causing the does-response contractile curve of KCl in K-H solution and CaCl2 in Ca-free K-H solution shifted to the rightward，and the values of their sensitivies and maximum contractions had been less. procyanidins could dose-dependently attenuate KCl elicited-contraction in rings， procyanidins induced-relaxation decreased significantly，after incubation with inhibitor of nitric oxide synthase Nω-L-nitro-arginine (L-NNA) or Methylene blue（MB）as well as endothelium removal. Conclusion：The results indicated that procyanidins relaxed vascular smooth muscle in an endothelium-dependent orindependent manners.The mechanisms for this phenomenon seemed to be related with promoting synthesis and release of NO as well as NO-cGMP pathway.The blockage of voltage-dependent calcium channels might underlie the main mechanism of its endothelium-independent vasorelaxation. 【Key words】Procyanidins；Coronary artery；Vascular tension；Endothelium；Voltage-dependent calcium channel 1947年，法国科学家杰克#8226;麦斯凯利博士首次于花生壳中分离原花青素（Procyanidins，PCs），后证实它广泛存在于植物中，是一大类多酚类化合物的总称，在抑制肿瘤、防癌抗癌、降脂[1，2]、抗凝血，防治心脑血管疾病常作为植物药源，近年研究集中于原花青素具有抑制亚硝基化、捕获和