大豆异黄酮对更年期大鼠抗氧化作用及提高学习记忆能力探究.doc

大豆异黄酮对更年期大鼠抗氧化作用及提高学习记忆能力探究【摘要】目的：观察大豆异黄酮对更年期雌性SD大鼠抗氧化作用及学习记忆的影响。方法：选用健康更年期雌性SD大鼠，随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水，实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮，连续60d。第60d取血测定血清过氧化脂质丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-PX)活力，用跳台实验方法测定大鼠学习记忆能力。结果：与空白对照组比较，中、高剂量组大鼠血清MDA含量显著降低，低、中、高剂量组血清SOD活力明显升高，中、高剂量组血清GSH-Px活力明显升高。结论：大豆异黄酮具有明显的抗氧化作用，并有效提高更年期雌性SD大鼠的学习记忆能力。 【关键词】大豆异黄酮；更年期大鼠；抗氧化；学习记忆 文章编号：1009-5519（2008）07-0954-03 中图分类号：R33 文献标识码：A Study of soybean isoflavones on antioxidative effect and improving the ability of study and memory in climacteric rats WANG Ai-mei1，ZHOU Jian-hui2，OUYANG Jing-ping1 （1.Medical College of Wuhan University，Hubei 430000，China；2.Nanyang Medical College of Henan，Henan 473058，China） 【Abstract】Objective：To investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.Methods：Forty-eight female SD climacteric rats were randomly divided into 4 groups with one control group and the other low，medium and high dose groups，which were given soybean isoflavones 0 mg/kg.bw，42 mg/kg.bw，83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.The levels of SOD，GSH-Px and MDA in serum were measured，and the ability of study and memory in each group was deterimined by adopting the law of diving platform.Results：The content of serum MDA in medium and high dose groups was lower than that in control group.The activity of serum GSH-Px im medium and high dose groups was significantly increased，so was the activity of serum SOD in low，medium and high dose groups.Conclusion：Soybean isoflavones do have the obvious effect of antioxidation，and improve the ability of study and memory effectively in female SD climacteric rats. 【Key words】Soybean isoflavon；Climacteric rat；Antioxidation；Study and memory 近年来国内外对大豆成分进行了广泛的研究，发现大豆皂甙具有抗氧化、降血脂等作用[1，2]；大豆异黄酮能有效预防癌症、骨质疏松等多种疾病的发生[3]。大豆异黄酮对更年期雌性大鼠抗氧化作用及学习记忆的影响少见报道，本研究采用更年期雌性大鼠，观察大豆异黄酮的抗氧化作用及对学习记忆能力的影响，为进一步开发利用提供科学依据。 1