川芎嗪对大鼠心脏缺血-再灌注损伤保护作用.doc

川芎嗪对大鼠心脏缺血-再灌注损伤保护作用[摘要] 目的：探讨川芎嗪对大鼠心肌缺血-再灌注损伤的保护作用。方法：SD大鼠30只，分为假手术组、模型组和川芎嗪治疗组，造模后分别于缺血30 min、再灌注60 min观察三组大鼠的血清CK、AST、LDH水平和心肌组织丙二醛（MDA）含量和超氧化物歧化酶（SOD）的活性变化。结果：模型组与对照组比较，CK、AST、LDH指标升高，心肌组织MDA含量和SOD活性升高，同治疗组相比，CK、AST、LDH水平升高，心肌组织MDA含量升高，SOD活性降低。结论：川芎嗪对大鼠心肌缺血-再灌注引起心肌损伤具有明显的保护作用，其机制可能与抑制自由基生成和清除氧自由基作用有关。 [关键词] 川芎嗪；心肌缺血-再灌注损伤；丙二醛；超氧化物歧化酶 [中图分类号] R285.5[文献标识码]A [文章编号]1673-7210（2009）03（c）-017-02 Protective effects of Ligustrazine on myocardial ischemia-reperfusion injury in rats ZHANG Qunfeng, CHEN Lianjian*, LI Min (Department of Pharmacy, the Futian Affiliated Hospital of Guangdong Medical College, Shenzhen518029, China) [Abstract] Objective: To study the protective effects of Ligustrazine on myocardial ischemia-reperfusion injury in rats．Methods: 30 SD rats were randomly divided into three groups: sham operative group (SG), model group (MG) and treatment group (Ligustrazine group, MG). The activities of creatinkinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in serum were determined, and activities of malondialdehyde (MDA) and superoxide dismutase (SOD) after myocardial ischemia 30 min and reperfusion 60 min. Results: compared with SG, the levels of CK, AST, ANF and LDH were increased, activities of MDA and SOD were increased in cardiac muscle on MG. Compared with TG, the levels of CK, AST, ANF and LDH in serum were increased, activity of MDA was increased and that of SOD was decreased in cardiac muscle. Conclusion: Protective effects of Ligustrazine on myocardial ischemia-reperfusion injury in rats is outstanding, which is possibly correlative with resisting and clearing SOD. [Key words] Ligustrazine; Myocardial ischemia-reperfusion injury; MDA; SOD 1 材料与方法 1.1 主要仪器与试剂 超氧化物歧化酶检测试剂盒、丙二醛检测试剂盒、蛋白检测试剂盒、DH-140B型动物人工呼吸机、全自动生化测定仪、721型可见分光光度计。 1.2 动物分组与模型建立 成年清洁级雄性SD大鼠30只，重250～300 g，由南方大学医学院实验动物中心提供，随机分为三组：假手术组、模型组、川芎嗪治疗组，大鼠腹腔注射戊巴比妥钠麻醉（30 mg/kg），经气管建立人工机械通气，呼吸频率65～70次/min。分离左侧股静脉插管用于药物的滴注。于胸骨左缘2～4肋间打开胸腔及心包膜，