瑞香素对垂体后叶素致大鼠急性心肌缺血保护作用探究.doc

瑞香素对垂体后叶素致大鼠急性心肌缺血保护作用探究[摘要] 目的：观察瑞香素对垂体后叶素致大鼠急性心肌缺血的保护作用。方法：采用大鼠舌下静脉注射垂体后叶素造成急性心肌缺血模型，心电图检测观察注射垂体后叶素后不同时间点的第Ⅱ导联心电图ST波变化百分率(%)，测定血清肌酸激酶同工酶(CK-MB)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量，并观察各项指标的变化。结果：瑞香素能明显对抗垂体后叶素引起的急性心肌缺血的心电图变化，提高血清SOD活性、降低CK-MB和MDA含量。结论：瑞香素具有显著对抗垂体后叶素致大鼠急性心肌缺血的作用。 [关键词] 瑞香素；垂体后叶素；心肌缺血 [中图分类号] R285.5[文献标识码]A [文章编号]1673-7210（2008）08（b）-019-02 Study of protective effect of daphnetin on acute myocardial ischemia induced by pituitrin in rats LI Hai-xia1, ZHANG Hong-ling1, DONG Bao-guo2, FANG Xiao-yan3 (1.College of Pharmacy, Zhengzhou University, Zhengzhou450052, China; 2.Technology Limited Company of Zhengzhou Times Flagship, Zhengzhou450008,China; 3.Henan Collage of Traditional Chinese Medicine, Zhengzhou450003, China) [Abstract] Objective: To investigate the protective effect of daphnetin on acute myocardial ischemia in rats. Methods:The models of acute myocardial ischemia were established by injecting pituitrin into the sublingual veins of rats. The percentage changes of ST wave at different time were determined by electrocardiogram(ECG). Changes of other indexes were evaluated by determining the creatine kinase isozymes (CK-MB), superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. Results: Daphnetin could significantly antagonize the electrocardiogram change of acute myocardial ischemia caused by pituitrin, raise the activity of SOD, and reduce the contents of CK-MB and MDA in serum. Conclusion: Daphnetin could effectively antagonize the acute myocardial ischemia induced by pituitrin. [Key words] Daphnetin; Pituitrin; Myocardial ischemia 瑞香素（daphnetin），化学名7,8-二羟基香豆素，是由我国创制的源于天然药物的新药[1]，临床上用于治疗血栓闭塞性脉管炎及其他闭塞性血管疾病和冠心病。本文采用舌下静脉注射垂体后叶素造成大鼠急性心肌缺血模型，观察瑞香素对心肌缺血的保护作用，并探讨其可能的作用机制。 1 材料与方法 1.1 实验材料与仪器 瑞香素，由本实验室提供，含量≥98% (HPLC)；垂体后叶素(Pit)注射液，每支10 U/ml，天津市生物化学制药厂生产；肌酸激酶同工酶MB（CK-MB），购于中生北控生物科技股份有限公司；超氧化物歧化酶(SOD)、丙二醛(MDA)试剂盒购于南京建成生物工程公司；BL-420S生物机能实验系统，成都泰盟科技有限公司生产；SHIMADZU紫外-可见分光光度计，日本生产。 1.2 分组及给药 雄性SD大鼠，体重240~280 g，由郑州大学医学院实验