癌基因STAT3及结直肠癌恶性程度关系探究.docVIP

癌基因STAT3及结直肠癌恶性程度关系探究摘要：目的：通过研究癌基因STAT3在结直肠癌及癌旁组织中 的表达，探讨其在结直肠癌发病机制中的作用。方法：采用免疫组化MaxVisionTM法检测40 例结直肠癌组织、37例癌旁组织和30例正常肠粘膜组织中STAT3蛋白的表达，并对结直肠癌 组织中STAT3蛋白的表达情况与不同临床病理特征进行统计学分析。结果：在结直肠癌和癌 旁组织中，STAT3蛋白的表达差异无显著性（P＞0.05）。相对于正常肠粘膜组织，结直 肠癌和癌旁组织的STAT3蛋白表达有显著差异（P＜0.05）。STAT3蛋白的表达水平与结 直肠癌的分化程度、临床分期及淋巴结转移有关（P＜0.05）。结论：STAT3高表达可能 是结直肠癌发病机制中的早期事件。在癌旁组织中，那些STAT3蛋白阳性的组织细胞可能是 潜在的癌前细胞。检测结直肠癌中STAT3蛋白的表达可以反映肿瘤的恶性程度。 关键词：癌基因；STAT3；结肠直肠肿瘤；免疫组织化学 中图分类号： R735.3+7;R446.8 文献标识码： A 文章编号： 1008-2409(2008)03-0419-02 The research on the relationship of oncogene STAT3 and m alignancy degree in colo rectal carcinoma/SHI Ren-fang，TANG Guo-duo，LI Yun-qian∥The Affiliated Hosp ital of Guilin Medical College， Guilin 541001， China Abstract: Objective: By researching the expression oncogene STAT3 in colorectal carcinoma (CRC) tissues and para-carcinoma tissues, to explore itsrole mechanism in tumorigenesis of CRC. Methods: The protein expressions of STAT 3 were detected in 40 CRC tissues, 37 para-carcinoma tissues and 30 normal colo rectal mucosa tissues by MaxVisionTM immunohistochemistry, and the relationshipof the expression of STAT3 in CRC with various clinicopatholoical characteristic s was analyzed statistically.Results: There was no significant difference betw een the expression of STAT3 protein in CRC tissues and para-carcinoma tissues ( P＞0.05). Compared with normal colorectal mucosa tissues, the expression ofSTAT3 protein in CRC tissues and para-carcinoma were significantly higher(P 0.05). Overexpression of STAT3 protein was correlated with differentiation, cl i nical stage and lymph node metastasis in CRC (P0.05). Conclusions:Overexp ression of STAT3 may be an early accidence in the pathogenesis of CRC. Inpara -carcinoma tissues, those STAT3-positive may be potential pre-cancerous cells . Determination of STAT3 protein can be used to indicate the m alignancy degree o f CRC.?Key words:oncogene；STAT3；colorectal neoplasm； immunohistochemistry 结直肠癌是临床最常见的恶性肿瘤之一。在全世界范围内，它是第四位常见的恶性肿瘤，