石榴皮中鞣花酸提取工艺探究.docVIP

石榴皮中鞣花酸提取工艺探究[摘要] 目的：考察石榴皮中鞣花酸的提取工艺。方法：选用4因素3水平正交表L9(34)，以薄层扫描法测定鞣花酸含量，考察4个因素（溶剂类型，提取时间，提取次数，溶剂用量）、3个水平对鞣花酸提取率的影响。结果：鞣花酸的最佳提取工艺为用1 mol/L的盐酸水溶液、8倍量、回流提取60 min、提取2次；结论：溶剂类型对鞣花酸的提取率影响最大，本研究确定的鞣花酸提取工艺合理、可行。 [关键词] 石榴皮；鞣花酸；提取工艺；正交试验 [中图分类号] R927[文献标识码]A [文章编号]1673-7210（2008）08（c）-050-02 Study on extract technology of ellagic acid in the peels of Punica granatum LI Hai-xia1,WANG Cai-fang1, ZHANG Hong-ling1, FANG Xiao-yan2,DONG Bao-guo3 (1. College of Pharmacy, Zhengzhou University, Zhengzhou450051，China;2. Henan College of Traditional Chinese Medicine，Zhengzhou450003，China;3.Technology Limited Company of Zhengzhou Times Flagship, Zhengzhou450008，China) [Abstract] Objective:To investigate the extract technology of ellagic acid in the peels of Punica granatum. Methods:The thin layer scanning method was used to determine the content of ellagic acid. The orthogonal design with three levels of four factors (the solvent, the extracting time, the extracting times and the amount of solvent) was adopted, using ellagic acid as index to evaluate the results. Results: The best extracting condition for ellagic acid was to add 8 times amounts of the water solution of 1 mol/L hydrochloric acid, and to reflux for 60 min and extract twice. Conclusion: The solvent has the maximal influence to the dissolutions of ellagic acid. The extract technology of ellagic acid was feasible and reasonable. [Key words] Peels of Punica granatum.;Ellagic acid;Extract technology;Orthogonal test 石榴皮为石榴科植物石榴（Punica granatum.L）的干燥果皮，在传统中药及临床上较为常用。《中华人民共和国药典》2005年版一部用没食子酸法对石榴皮中的总鞣质进行了含量测定，而鞣花酸(ellagic acid, EA)为石榴皮中的有效成分之一，具有抗癌、抗菌、抗病毒、抗氧化等作用[1]。石榴皮中富含生鞣花酸，并且在酸性条件下水解可产生更多的鞣花酸，本文利用正交试验优选石榴皮中鞣花酸的提取工艺，为今后科学利用和开发石榴皮奠定基础。 1仪器与试剂 1.1仪器 JT2002型电子天平（上海精天公司），CAMAG-Ⅱ型薄层扫描仪，RE-52A旋转蒸发器(上海亚荣生化仪器厂)，SHZ-D循环水式真空泵(巩义市英峪予华仪器厂)。 1.2试剂与材料 石榴皮药材(购自河南郑州，由河南中医学院刘若庸教授鉴定)，鞣花酸对照品为自制（经1HNMR、13CNMR光谱鉴定），丙酮、乙醇、盐酸等其他所用试剂均为分析纯。 2方法与结果 2.1正交实验设计 鞣质极性较强，故在正交实验中选用极性较大的溶剂(A)：70%丙酮、1 mol/L HCl水溶液、95%