冷冻干燥同种异体髂骨支架材料的制备和性能研究张泽华1罗飞1.DOC

冷冻干燥同种异体髂骨支架材料的制备和性能研究 张泽华1，罗飞1，张劲松1，曾玲1，许建中1，陈卫军2 1第三军医大学西南医院骨科，重庆，400038重庆，400038方法大体特征扫描电镜观察，力学性能。结果0.35±0.03g/ml，孔隙率67.23±9.12%，孔径395.36±37.02 um，抗扭强度5.96±1.53 MPa，抗压强度9.94±0.73 MPa，弹性模量376.14±27.32 MPa，抗弯强度 15.70±2.35 MPa。结论rocessing and Property Study of Freeze-Dried Iliac Crest Bone Allograft ZHANG Ze-hua, LUO-fei, ZHANG Jin-song, etal Department of Orthopaedics, the Southwest Hospital, Third Military Medical university, ChongQing, 400038 Biomechanics Section Under the Ministry of National Education’ Key Laboratory of Biomechanics and Tissue Engineering, Third Military Medical university, ChongQing, 400038 Abstract Objective: To study the microstructure and biomechanical properties of freeze-dried iliac crest bone allograft for further application as bone substitute, bone tissue engineering scaffolds and drug delivery system. Methods: Procurement, processing, sterilization and conservation of the iliac crest allograft scaffold were all performed according to the guidelines of the American Association of Tissue Banks. Its physical characteristics were observed including colour, texture and porosities, the diameter of pores and microstructure were tested with scanning electron microscope．Its porosity and density were tested with liquid displacement technique. Biomechanical tests were conducted in axial compression, lateral bending and rotation modes. Result: The freeze-dried iliac crest bone allograft was ivory white, hard , clean and porous, presenting sandwich structure with tricortical and cancellous bone. Its mean density ,porosity and pore diameter was 0.35±0.03g/ml, 67.23±9.12% , 395.36±37.02 um,respectively. The mean anti-rotation strength, compressive strength, elastic modulus , buckling strength was 5.96±1.53 MPa, 9.94±0.73 MPa, 376.14±27.32 MPa, 15.70±2.35 MPa, respectively. Conclusion: The freeze-dried iliac crest bone allograft was a natural porous bone substitute with satisfying biomechanical strength, it’s facing a bright prospect in further st