吲哚美辛对大鼠胃功能的影响及作用机制研究.PDFVIP

吲哚美辛对大鼠胃功能的影响及作用机制研究 刘静1 1,2 2 1* ，胡金芳 ，刘兆凤 ，申秀萍 (1.天津药物研究院，天津 300193 ；2.天津中医药大学，天津 300193) 摘要：目的 研究吲哚美辛对大鼠胃酸分泌及胃蛋白酶活力的影响及其作用机制。方法 大鼠分为对照组和吲哚美辛低、 中、高剂量组。测定各组大鼠胃酸分泌量及胃蛋白酶活力，测定血清胃泌素含量。结果 吲哚美辛可显著增加胃酸分泌 量、增加胃酸分泌速度及增加胃蛋白酶活力，促进胃泌素分泌。结论 吲哚美辛可显著促进胃酸分泌，增加胃蛋白酶活 力，这与其促进胃秘素分泌有关。 关键词：吲哚美辛；胃溃疡；胃蛋白酶；胃泌素；胃酸分泌 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2011)12-1074-03 Effects of Indomethacin on Function of Stomach in Rats and its Mechanism LIU Jing1 1,2 2 1* , HU Jingfang , LIU Zhaofeng , SHEN Xiuping (1.Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China; 2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China) ABSTRACT: OBJECTIVE To study the effects of indomethacin on function of stomach in rats and its mechanism. METHODS Rats were randomly divided into control group, low-, middle- and high-dose of indomethacin groups. The gastric acid secretion, protease activity and serum gastrin levels were determined. RESULTS Compared with control group, indomethacin significantly increased gastric acid secretion, increased the rate of gastric acid secretion and increased pepsin activity, and promoted the secretion of gastrin. CONCLUSION Indomethacin could promote gastric acid secretion, increase pepsin activity, which facilitate their secret factors of gastric secretion. KEY WORDS: indomethacin; gastric ulcer; pepsin; gastrin; gastric acid secretion 吲哚美辛等非甾体抗炎药(NSAIDs)是一类具 由北京维通利华实验动物技术有限公司提供，实 有抗炎、镇痛作用的药物，其临床应用广泛。但 验动物许可证号：SCXK(京)2007-0001 。动物饲 长期大量服用容易引起对胃肠道的损害[1] ，可引起 养在天津药物研究院实验动物屏障系统(津实动 消化不良、胃肠道黏膜糜烂、消化性溃疡和出血[2] ， 设施准第 012 号) ，实验动物使用许可证号： 甚至危及生命。文献[3-5]表明吲哚美辛等 NSAIDs SYXK( 津 )2006-0002 。设施环境温度维持在 引起胃溃疡的机制主要与其抑制环氧合酶，减少 2