胰岛素样生长因子-1在骨性ⅲ类错合畸形中的表达-第三军医大学学报.doc

胰岛素样生长因子-1在骨性Ⅲ类错合畸形中的表达研究 陈允嘉 李艳 秘双燕 吴增波 王强 王豫蓉* （重庆医科大学附属口腔医院上清寺院区正畸科 400015） 摘要RT-PCR检测结果显示，骨性Ⅲ类错合畸形组中IGF-1mRNA的表达量是5.5410±2.0447μgμl，骨性Ⅰ类组中IGF-1mRNA的表达量是1.2821±0.2731 μgμl，两组比较具有统计学差异（P＜0.05）。ELISA检测结果显示，骨性Ⅲ类错合畸形组中IGF-1蛋白的表达量是84.1259±29.2947 μgl，骨性Ⅰ类组中IGF-1蛋白的表达量是22.4064±4.9312μgl，两组相比具有显著差异（P＜0.05）The expression of Insulin-like Growth Factors-1 in class Ⅲ malocclusion Chen Yunjia ，Li Yan，Mi Shuangyan，Wu Zengbo，Wang Qiang，Wang Yurong*. *Department of Orthodontics，Hospital of Stomatoligy，Chongqing medical university，Chongqing 400015，China Corresponding auther：Wang Yurong，E-mail：rywmin66@，TelAbstract: Objective To approach the expression of IGF-1 between Class Ⅲ malocclusion and ClassⅠmalocclusion. Moreover, we will investigate the relationship between the expression of IGF-1 and the mandibular growth. Methods 34 patients of skeletal class Ⅲmalocclusion and skeletal class Ⅰmalocclusion were included, Detect the expression of IGF-1mRNA and IGF-1 protein of patients in Class Ⅲ malocclusion and ClassⅠmalocclusion by Real Time polymerase chain reaction (PCR) and Enzyme Linked Immunosorbent Assay (ELISA) method . Result The detection result of RT-PCR method showed that the expression of IGF-1mRNA in Class Ⅲ malocclusion group is 5.5410±2.0447μg/μl ,which was significantly higher than that in ClassⅠmalocclusion group. Elisa assay showed the expression of IGF-1 protein in Class Ⅲ malocclusion group and ClassⅠmalocclusion group was 84.1259±29.2947μg/l and 22.4064±4.9312μg/l，respectively. There was significant deviation between the two group(P＜0.05) . Conclusions The more mandibular growth capacity , the higher the expression of IGF-1. So the IGF-1 played an important promotion in the growth mandibular condyloid process. Key words: Class Ⅲ malocclusion，IGF-1，mandible，genetic factorIKuo Yonemitsu【9】等人的实验证实：IGF-1基因能加速髁状突软骨成骨的速度和阻止髁状突软骨细胞凋亡，因此可将其作为观察下颌骨生长的一个指标。T．Yolota【10】等人的研究发现，在大鼠下颌骨髁状突的未分化间质细胞层中检测到IGF-1基因的表达，并且证实IGF-1基因是调节细胞凋亡的一个重要内源性因素。这些研究表明IGF-1基因与下颌骨的生