硬脑膜外记录视皮层电活动的试验研究-第三军医大学学报.doc

硬脑膜外记录视皮层电活动的实验研究 刘娜1，石卫卫2，陈丽峰1，侯文生2，阴正勤1* 1第三军医大学西南医院/西南眼科医院，重庆 400038 2重庆大学生物工程学院，重庆 400030 　*通讯作者 摘要　 目的　建立初级视皮层区硬脑膜外柔性微电极阵列记录视皮层电活动的方法及实验平台，为研究硬脑膜外视皮层假体奠定实验基础。 方法　麻醉麻痹状态下的成年家猫，自行设计的柔性微电极阵列，微创植入并固定于视皮层定位区的硬脑膜外，给予闪光和图形视觉刺激，记录视觉诱发电位。 结果 　可通过微创手术将微电极阵列植入并固定在视皮层定位区的硬脑膜外；在硬脑膜外微电极阵列可记录到有效VEP；黑白棋盘格图形刺激优于黑白闪烁刺激。 结论 在视皮层定位区硬脑膜外首创的微创植入并固定柔性微电极阵列的方法适用性、重复性好，经硬脑膜外利用微电极阵列记录诱发电位是可行的，可作为基于视皮层硬脑膜外电刺激视觉修复假体的实验基础。 关键词 硬脑膜外，微创手术，柔性微电极阵列，视皮层假体，视皮层电活动　 The experimental study on visual cortex electric activity on the epidural area Liu Na 1 , Shi Weiwei 2, Chen Lifeng 1 , Hou Wenshen 2, Yin Zhengqin 1* 1 Southwest Hospital / Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, China 2 Chongqing University, Chongqing 400030, China * Corresponding author　 Abstract Objective 　A new experiment method for studying visual cortex electric activity by the flexible micro-electrode array implanting on the duramater above visual cortex, to establish the experiment basis for studying visual cortex prosthesis in the epidural area. Methods All adult cats were anesthetized, and the flexible micro-electrode array designed by our group was implanted on the epidural area within the visual cortex location region．The flash light and checker reversal stimulation were taken and VEPs were recorded. Results The micro-electrode array could be implanted on the epidural area within the visual cortex location region by the minimally invasive surgery; Valid VEP record could be got by the flexible micro-electrode array in the epidural area; VEPs evoked by checker reversal stimulation easier than those by flash light stimulation. Conclusion The patent operative procedure of the minimally invasive surgery was good adaptivity and reproducibility. It is feasible to record VEP by implanting the flexible micro-electrode array on the epidural area; It can be used as the experimental basis for stimulating visual prosthesis on the epidural area within the visual cortex region.　 Keyword