罗库溴铵对大鼠正常或损伤面神经与肢体神经肌接头突触前膜 .doc

麻醉学基础研究 中青年优秀论文评奖 罗库溴铵对大鼠正常或损伤面神经与肢体神经肌接头突触前膜乙酰胆碱量子式释放的影响 陈君亮1，陈莲华2 1发言作者：复旦大学附属眼耳鼻喉科医院麻醉科，上海，200031； 2通讯作者：上海交通大学附属第一人民医院麻醉科，上海，200080 通信作者：陈莲华（E-mail:chenlianhua@citiz.net）【摘要】目的 比较大鼠正常面神经和神经肌接头在不同浓度罗库溴铵作用下突触前膜乙酰胆碱释放的量，观察正常或面神经和肢体神经支配的肌肉对罗库溴铵的敏感性差异是否与三种神经肌接头突触前膜神经递质释放功能有关。方法 采用自体对照设计，制备大鼠右侧面神经主干急性压榨伤模型，左侧为正常对照侧；分别取双侧面神经主干及相连接的口轮匝肌肌条和肢体神经主干及相连接的腓肠肌肌条，用细胞内微电极记录法测定三种神经肌接头的终板电位（EPP）和微终板电位（MEPP）；分别在相当于大鼠0.5ED95、ED95、1.5ED95、2ED95血药浓度的罗库溴铵梯度浓度孵化下记录EPP和MEPP，比较正常面神经、面神经和神经突触前膜乙酰胆碱量子式释放（EPP/MEPP）的差异。结果 三种神经肌接头的EPP都随罗库溴铵浓度增加在不同浓度罗库溴铵水平下））随罗库溴铵浓度增加μg /ml浓度罗库溴铵水平下在5μg /ml、7.5μg /ml和10μg /ml罗库溴铵水平下）结论 罗库溴铵对正常或面神经和肢体神经肌接头突触前膜释放功能对罗库溴铵敏感性差异【关键词】罗库溴铵effect of rocuronium on presynaptic quantal release of acetylcholine from normal or damaged facial nerve and somatic nerve innervated neuromuscular junctions in rats CHEN Jun-liang1, CHEN Lian-hua2 1Department of Anesthesiology, The Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China 2Department of Anesthesiology, The affiliated first people’s hospital, Shanghai Jiaotong University, Shanghai, 200080, China Presenting anthor: CHEN jun-liang Corresponding author: CHEN lian-hua, Email: chenlianhua@citiz.net [Abstract] Objective To compare the amount of presynaptic quantal release of acetylcholine from normal or damaged facial nerve and somatic nerve innervated neuromuscular junctions under the effect of different concentrations of rocuronium in rat in vitro. To evaluate whether the differences in sensitivity to rocuronium among the normal or damaged facial nerve and somatic nerve innervated muscles are related to the function of presynaptic neurotransmitter release. Methods Autologous paired controlled design was used in the study. An acute nerve trunk squeeze injury model was induced in the right facial nerves of the rats and the left facial nerves were used for control. The facial nerve trunks from both sides with the connected orbicularis oris muscle strips and the somatic nerve trunks