基础研究补肾中药对成骨细胞vdr、cbf1mrna表达的.pdf

2006 11 19 11 C h ina J Or hop T rauma, N ov. 2006, V ol. 19, N o. 11 659 VDRCbf1mRNA 1 2 2 3 魏义勇 , 石印玉, 詹红生, 冯伟 ( 1, 7 100 32; 2 ; 3) ! : D ( V DR ) 1( Cbf1) mRNA ( O B) , VDRCbf1m RNA , : 6, , RTPCR VDRCbf1m RNA : RTPCR , , VDRCbf1m RNA ; VDRCbf1mRNA VDRCb f1m RNA : VDRCbf1m RNA , ; VDRCbf1m RNA , ! ; ; Effects of reinforcing K idney Chinese medicine herbal( ) on expression of VDR, Cbf1mRNA * * in rat osteoblast WEI Y iyong , SH I Y inyu, ZH AN H ong sh eng, FEN G We .i Inst itute of O rth op aed ics, X i ing H osp ita l, th e FourthM il itary Un iv ersity, X ia n 710032, Sh anx i, Ch ina A STRACT Objective:T o inves iga e he effec s o f reinforcing K idney Ch inese herba lmed ic ine such as GubenZ h ua ngg u ( ) , J ingu iS h enq i( ) , B ushenY i ing () , Z h iba iD ihuang ( ) on expression of VDR, Cb f1m RNA and explore he underly ing m echanism s. Methods: O s eob las o f 6 m on hage ra s w as iso la ed and cu lured by he issuefragm en m ig ra ingg row h m e hod. B io log ica l charac eris ic w as observ ed by phasecon ras m icroscope, m inera lizing nodule s ain ing echnique and elec rom icro scope. T he expression o fV DR, Cbf1m RNA w as de ec ed by RTPCR. Results:RTPCR show ed ha GubenZ huangg u cap sule, J ingu iS h enq i p ill upregu la ed he express ion w h ich w as he sam e as Boneone and Z h iba iD ihua ng p ill dow nregula ed he express ion of VDR, Cbf1m RNA in ra os eob las, bu he expression w as d ifferen in B ush enYi ing decoc ion. Conclusion:GubenZ huangg u cap sule and J ingu iS h enq i p ill promo ed bone forma ion by upregu la ing he expression of VDR, Cbf1m RNA bu Zh iba iD ih ua ng p ill inh ib i ed he bone fo rm a ion by dow nregu la ing he expression. K ey words K idn