菌株DN2对烟草薄片制备液中烟碱的降解.PDF

生 物 工 程 学 报 Chin J Biotech 2009, June 25; 25(6): 897-902 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 工业生物技术 DN2 1,2 2 1 袁勇军 , 陆兆新 , 戚向阳 1 , 315100 2 , 210095 : 使用 O. intermedium DN2 降解烟草薄片制备液中的烟碱。研究了各种工艺参数对烟碱降解的影响, 单因素考察 结果表明烟草薄片制备液中烟碱降解的最适条件是: 添加0.1%的酵母膏, 使用氨水将pH 调节到7.0, 接种 15%种子液, 培养温度 30oC 。在上述条件下, 采用 30L 发酵罐对烟草薄片制备液进行 3 个批次的半连续发酵, 烟碱的平均降解速率 为 140.55 mg/L/h, 高于其他烟碱降解菌株。该结果表明菌株DN2 可以用来降低烟草薄片中的烟碱含量。 : 烟草薄片, O. intermedium DN2, 烟碱, 生物降解 Biodegradation of nicotine in tobacco extracts for making reconstituted tobacco by strain DN2 1,2 2 1 Yongjun Yuan , Zhaoxin Lu , and Xiangyang Qi 1 College of Biological Environmental Science, Zhejiang Wanli University, Ningbo 315100, China 2 College of Food Science Technology, Nanjing Agricultural University, Nanjing 210095, China Abstract: The purpose of the study is to use O. intermedium DN2 to degrade nicotine in tobacco extracts for making reconstituted tobacco. Firstly, we studied the effects of various factors on degradation of nicotine in the extracts by strain DN2. When we added 0.1% yeast extract into the extracts, adjusted its pH value to 7.0 by ammonia solution, inoculated 15% cultures and maintained fermentation temperature of 30°C, the degradation rate of nicotine by strain DN2 was the fastest. Furthmore, under these conditions, we studied the degradation rates of nicotine in three fed batches culture whi