外源果糖、葡萄糖与一氧化氮供体（SNP）对玉米种子萌发的影响.pdfVIP

玉 米 科 学2008，16(3)：74—77，81 Journal of Maize Sciences 文章编号：1005—0906(2008)03—0074—04 外源果糖、葡萄糖与一氧化氮供体(SNP) 对玉米种子萌发的影响 徐勤虎，董树亭，高荣岐 (山东农业大学农学院，山东 泰安 271018) 摘 要：测定了一氧化氮供体(SNP) 葡萄糖(G)、果糖(F)单独或组合浸种发芽特性，探讨了玉米种子萌发 过程中信号传导途径。结果表明，各处理不同程度提高了玉米种子发芽指数、根长、苗高、根干重、苗干重、8一 淀粉酶活性和早期萌发率，通过测定萌发早期不同时间段种子含水量证实，不是由于促进种子吸胀引起的。进 一 步用果糖、葡萄糖分别与SNP混合后浸种，G与SNP单独浸种在各项指标中表现出相同的趋势，相对于G、 SNP单独处理，G+SNP对苗干重和根干重影响小于二者单独处理且差异显著。F对各项指标的影响弱于SNP； 相对于F，F+SNP浸种提高了根和苗的干重。G+SNP浸种对 8一淀粉酶活性的影响相对于单独处理具有趋中的 趋势，而F+SNP则低于二者单独处理。表明葡萄糖与一氧化氮可能存在相同的信号途径，而果糖则有另外的信 号通路。 关键词：玉米种子；萌发；一氧化氮；糖信号；信号传导 中图分类号：$513．01 文献标识码：A The Effects of Exogenous Glucose，Fructose and NO Donor Sodium Nitroprusside(SNP)on Maize Seed Germination XU Qin-hu，DONG Shu-ting，GAO Rong-qi gronomy ofShandong Agricultural University，Taian 271018，China) Abstract：The effects of seed soaking with exogenous glucose(G)，fructose(F)and NO donor(SNP)alone or compounded on seed germination of maize were tested to explore signal~ansduction pathway in seed germination． The resuhs showed that the treatments could improve early germination rate，germinating index，root length，shoot length root，dry weight and 8-amylase activities．The resuhs of water content determination of maize in different time，which seed soaking with exogenous glucose，fructose and nitric oxide(NO)donor sodium nitroprusside(SNP)， showed that it had no relationship with seed imbibitions．Effects of exogenous glucose and fructose combined with SNP on the maize seed germination and seedling growth were surveyed．Soaking with exogenous glucose G had the same trend in indicators；Compared with G，SNP pretreatment，G+SNP pretreatment had a significantly weaker impact on shoot dry weight and