外周血CD5^＋ B细胞与HBV感染慢性化、严重化的关系.pdfVIP

丕医 2008年第48卷第l6期 外周血CD；B细胞与HBV感染慢性化、 严重化的关系 陈士俊 。陈文娟 。徐可树h (1华中科技大学同济医学院附属协和医院，湖北武汉430022： 2济南市传染病医院；3山东大学医学院) [摘要] 目的 探讨co；B细胞是否参与乙型肝炎的慢性化及严重化进程。方法 将慢性肝病患者分为慢 性乙型肝炎、慢性重型肝炎、肝炎后肝硬化三组，并设健康对照。检测肝脏各项检测指标(丙氨酸转移酶、天冬氨酸 转移酶、总胆红素、间接胆红素、碱性磷酸酶、 -谷氨酰转肽酶、白蛋白、球蛋白、凝血酶原活动度、胆固醇及HBV． DNA定量)，应用流式细胞仪检测外周血c B细胞百分率。结果 肝硬化组co；B细胞百分率明显高于对照 组；慢性乙肝组co；B细胞百分率与凝血酶原活动度有相关性(r=0．457，P0．05)，与肝功能其他各项指标均无 相关性；其余各组与肝功能各项指标均无相关。结论 cD B细胞可能参与了乙型肝炎慢性化进程，但与病情严重 程度无关。 [关键词]CD[B细胞；肝炎，乙型；流式细胞术；肝功能试验 [中图分类号] R512．6 [文献标识码] A [文章编号] 1002-266X(2008)16-0018-03 Relation between co；B cells jn peripheral blood and the chronic and severe process of hepatitis B CHEN Shi-jun ，CHEN Wen-juan，XU ICe．shu (1 Affiliated Xiehe Hospital，Huazhong University ofScience and Technology，Wuhan 250012，P．R．China) Abstract：Objective To investigate whether CD5+B cells participated in the chronic and severe progress of hepatitis B．Methods Chronic hepatitis patients were divided into three groups(chronic hepatitis B，sever chronic hepatitis B and hepatic cirrhosis)，15 out-patient clinic healthy volunteers were taken as control group．Liver indexs(ALT，ST，TB，TD， ALP， -GT，A，G，PrA，CHO，HBV-DNA)were detected and the rate ofCD；B cells WaS detected by flow Cytometry Results The rates of CD5+B cells of hepatic cirrhosis group were obviously higher than that of control group；the rate of CD；B cells of chronic hepatitis B patients haS positive relation with PTA，but there WaS no relation with otherliverfunction；in 8ev- er chronic hepatitis B and hepatic cirrhosis patients，there WaS no relation with every liver index．Conclusions CD5+B cells may participate in the chronic process ofhepatitis B，but have nO relation with the severe progess ofhepatitis B． Key words：CD5+B cell；hepatitis B；flow cytometry；liver function tests CD；B细胞分泌许多自身抗体及抗病原菌的抗