幽门螺杆菌培养及抗原蛋白的分离纯化.doc

ISSN 1009-3079 CN 14-1260/R 世界华人消化杂志 2008年4月8日;16(10): 1098-1104 文献综述 REVIEW 幽门螺杆菌培养及抗原蛋白的分离纯化赵圣国, 王加启, 刘光磊, 程金波, 张春刚 ? 赵圣国, 王加启, 刘光磊, 程金波, 张春刚, 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室 北京市 100094国家“十一五”科技攻关奶业重大专项资助项目, No. 2006BAD04A03作者贡献分布: 赵圣国对此文做主要贡献; 此课题由王加启提出设计; 刘光磊, 程金波和张春刚对本文修改和审阅; 文章思路及写作由赵圣国完成.通讯作者: 王加启, 100094, 北京市圆明园西路2号, 中国农科院北京畜牧兽医研究所. wang-jia-qi@263.net电话: 010收稿日期: 2007-09-13 修回日期: 2008-02-27Culture of Helicobacter pylori and purification of antigen protein Sheng-Guo Zhao, Jia-Qi Wang, Guang-Lei Liu, Jin-Bo Cheng, Chun-Gang ZhangSheng-Guo Zhao, Jia-Qi Wang, Guang-Lei Liu, Jin-Bo Cheng, Chun-Gang Zhang, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100094, ChinaSupported by: the Dairy Momentous Project of National Key Technology RD Program during the 11th Five-Year Plan Period, No. 2006BAD04A03Correspondence to: Jia-Qi Wang, Institute of Animal Science, Chinese Academy of Agricultural Science, 2 Yuanmingyuan Western Road, Beijing 100094, China. wang-jia-qi@263.netReceived: 2007-09-13 Revised: 2008-02-27 AbstractHelicobacter pylori (H pylori) live in the stomach of human. It is a kind of curve bacteria which can lead to chronic gastritis, peptic ulcer and gastric cancer. Usually, antibiotics are used to treat the patients, but they have a lot of side effects. Milk of milch cow immunized with H pylori vaccine can prevent and treat the infection of H pylori without side effects. During the production of immune milk, the basic work is to prepare vaccine. During the preparation of sub-unit vaccine, key is to isolate and purify the antigen protein. In this article, we discuss the culture of H pylori (solid and liquid culture), the recombinant expression and purification of its antigen protein (urease, VacA, CagA and lipopolysaccharide).Key Words: Helicobacter pylori; Culture; Urease; Vacuolization cytotoxin A; Cytotoxin associated antigen A; Lipopolysacchari