薄层扫描法测定乐口颗粒剂中小檗碱含量（Determination of lekou granules by TLC the content of berberine hydrochloride）.doc

薄层扫描法测定乐口颗粒剂中小檗碱含量（Determination of lekou granules by TLC the content of berberine hydrochloride） Determination of lekou granules by TLC the content of berberine hydrochloride Update: 2010-11-19: click on the Hui Sha Wang Huaiyou Cao Yanhua Qiu The content of berberine hydrochloride in lekou granules was determined by TLC scanning method. The determination results of sample RSD is 2.87%; the recovery rate and precision were studied. Keywords TLC scanning; lekou granules; Berberine TLC-Densitometric Determination of Berberine in Lekou Granules Hui Qiusha, Wang Huaiyou, Cao Yanhua Shandong University of Traditional Chinese Medicine, Jinan 250014 Abstract A method for the determination of Berberine by TLC-Densitometry in Lekou granules is reported. RSD of the three results is 2.87%. At the same time, recovery and accurance is also studied. Key words TLC-Densitometry; Lekou Granules; Berberine Lekou granules from Phellodendron and other drugs, with a clear fire bushenzhiyi function is the treatment phase fire caused by fine classical lost dream. Modern clinical experience shows that this method in the treatment of fire attack caused by oral ulcer, mouth sores, the effect is particularly significant. To control the quality of products, the application of TLC scanning method for the determination of berberine in lekou granules. 1 instruments and reagents CS-930 dual wavelength TLC scanner (SHIMADZU), quantitative capillary (SHIMADZU), AE240s electronic analytical balance (Switzerland, MEYTLER); berberine hydrochloride (Chinese control of pharmaceutical and Biological Products Institute), lekou granules (homemade); reagents were analytically pure. Methods and results 2 2.1 determination conditions 2.1.1 sample preparation: sample 5 g, weighing precision, the Soxhlet extractor, with ether degreasing 130 ml 1 h, volatile ether, and methanol extract 130 ml 5 h, the recovery of methanol to about 8 ml, filtration, the filtrate was concentrated in low temperature place, and set