humanIFN-γ血清正向.docVIP

Human Interferon g (IFN-g) ELISA Kit 48 Tests Catalogue Number: E01I0345 Store all reagents at 2-8°C Valid Period: six months For samples: Serum or blood plasma FOR LABORATORY RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! INTENDED USE This BG IFN-g ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-g ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve. PRINCIPLE OF THE ASSAY This IFN-g enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific for IFN-g. Standards or samples are then added to the microtiter plate wells and IFN-g if present will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFN-g present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFN-g are added to each well to “sandwich” the IFN-g immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-g and enzyme-conjugated antibody will exhibit a change in color. The enzyme-subst