CTGF在烟雾暴露大鼠肺血管中的表达及其与肺血管重建的关系.pdfVIP

虫国痘理生理 蠢 Chinese Journal of Pathophysiology 2008，24(1)：7—1 1 · 7· [文章编号] 1000—4718(2008)01—0007—05 CTGF在烟雾暴露大鼠肺血管中的表达及其 与肺血管重建的关系 田 锋 ， 徐永健 ， 张珍祥 ， 胡 静 ， 范新蕾 ( 华中科技大学同济医学院附属同济医院呼吸内科，湖北武汉430030； 山东医学专科学校内科，山东 临沂276000) [摘 要] 目的：探讨烟雾暴露致大鼠肺血管重建中结缔组织生长因子(CTGF)表达的变化及意义。方法： 24只大鼠随机分为对照组、烟雾暴露1、2和3月组，HE染色观察肺血管重建，天狼猩红染色检测胶原增殖，免疫组 化观察CTGF在肺动脉中的表达，RT—PCR检测肺动脉CTGFmRNA表达。结果：随烟雾暴露时间延长，肺动脉管 壁面 管总面积(WA％)、胶原沉积、CTGFmRNA和蛋白表达呈时间依赖性增加(组间P0．01)。CTGF表达与 WA％呈正相关。结论：大鼠烟雾暴露后早期即出现肺血管重建，且随时间延长逐渐加重，CTGF在肺动脉中的表达 逐渐升高，可能在此过程中起重要作用。 [关键词] 结缔组织生长因子；烟雾；肺动脉；肌，平滑，血管 [中图分类号] R363 [文献标识码] A Relationship between connective tissue growth factor expression in pulmo- nary arteries and pulmonary artery remodeling induced by smoke TIAN Feng ，XU Yong—jian ，ZHANG Zhen—xiang ，HU Jing ，FAN Xin—lei ( Department ofRespiratory Medicine，torsi Hospital，Tongji Medical College，Huazhong University ofScience and 一 nology，Wulmn 430030，China； Department ofInternal Medicine，Shandong Medical College，tinyi 276000，China．E— mail：yjxu@tjh．tjrnu．edu．cn) [ABSTRACT] AIM：To study the relation between smoke—induced pulmonary artery remodeling and connective tissue growth factor(CTGF)expression in pulmonary arteries．METHODS：Twenty—four male Wistar rats were randomly assigned into control group and smoke exposure group，and the latter was further divided into three time points of 1，2，3 months．Pulmonary artery remodeling was observed by Hematoxylin—Eosin staining，and collagen contents were investiga— ted by sirius red staining．Immunohistochemistry methods were performed to determine CTGF protein expression in pulmo— nary artery smooth muscle．The expressions of CTGF mRNA in pulmonary arteries were evaluated by RT—PCR．RE- SULTS：(1)The pulmonary artery WA％ in four gr