ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制.pdfVIP

第3O卷第5期 第 三 军 医 大 学 学 报 Vo1．30，No．5 2008年3月 ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE Mar．2008 393 文章编号：1000-5404(2008)05—0393—03 ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制 郭 晓，潘 岐 (河北医科大学第二临床医院血液科，石家庄050000) 摘 要：目的 研究细胞外信号调节激酶(extracellular signal—regulated kinase，ERK)阻滞剂U0126对K562细胞周期 的作用及机制。方法 以逆转录一聚合酶链反应(reverse transcription—polymerase chain reaction，RT．PCR)和Western blot检 测U0126处理K562细胞后ERK、Cyclin D2、Cyclin E、P27 mRNA和蛋白的表达；以流式细胞术(flow cytometry，FCM)检测 细胞周期的变化。结果 处理后K562细胞ERK、Cyclin D2、Cyclin E mRNA和蛋白表达降低；P27 mRNA和蛋白表达增 高。G。／G。期细胞增多，s期细胞减少，与用药前相比差异均有统计学意义(P0．05)。结论 U0126可能通过ERK途 径，影响细胞周期调控蛋白，最终抑制了K562细胞的增殖。 关键词：白血病；细胞周期；ERK；K562细胞；U0126 中图法分类号：R329．28；R73-361；R733．7 文献标识码：A Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines GUO Xiao，PAN Ling(Department of Hematology，the Second Hospital of Hebei Medical University，Shijiazhuang 050000，China) Abstract：Objective To study the mechanism of extraeellular signal—regulated kinase(ERK)pathway inhibitor U0126 on cell cycle of K562 cell lines．Methods The mRNA and protein expressions of ERK．Cyc— lin D2，Cyclin E in and P27 K562 cell lines after 10 mol／，L U0126 treatment for 12，24 and 72 h were detec— ted by RT—PCR and Western blot，respectively．The cell cycle was determined by flow cytometry．Results The mRNA and protein expressions of ERK，Cyclin D2 and Cyclin E in K562 cell lines after U0 1 26 treatment were decreased，while those of P27 were increased．The percentage of cells in G0／Gl phase was increased and that in S phase was decreased．All data mentioned above showed significant difference between the K562 cell lines before and after U0 1 26 treatment(P0．05)．Conclusion U0 1 26 possibly affects cell cycle regulating proteins by ERK pathway and eventually inhibits the proliferation of K562 cells