交联温度对京尼平交联胶原壳聚糖组织工程支架的影响.docVIP

交联温度对京尼平交联胶原/壳聚糖组织工程支架的影响来源：中国论文下载中心????[ 10-05-04 09:57:00 ]????编辑：studa20 ????? ?????????????? 作者：曹峥，胡蕴玉，潘纬敏，白雪东，毕龙，李丹，杨小彬，刘民 【摘要】? [目的]通过研究材料的孔径、交联度、溶胀率、降解率、细胞毒性及组织相容性的变化，探讨交联温度对京尼平交联胶原/壳聚糖支架的影响。[方法] 采用冷冻干燥法制备胶原/壳聚糖复合多孔支架，分别于4、20、36条件下，在0.5%京尼平水溶液中交联24 h。以未交联的胶原/壳聚糖复合多孔支架作为对照，评价所得支架的孔径、交联度、溶胀率、降解率、细胞毒性及组织相容性特点。[结果]随交联温度升高，支架的交联度明显增大，溶胀率和降解率逐渐减小。4组支架交联度47.88%±6.4%，溶胀率为721%±46%，4周后降解3.95%±6.4%;20组支架交联度67.69%±3.6%，溶胀率为662%±72%，4周降解0.91%±5.9%;36组支架交联度70.32%±5.7%，溶胀率为635%±27%，4周降解0.66%±7.3%，三组上述观察指标均优于未交联组(P/0.01)。[结论]京尼平交联可以显著降低胶原/壳聚糖支架的降解率和溶胀率，且对支架结构和生物相容性无明显影响。交联温度增加可以提高支架的交联度和抗降解能力，较快获得具有良好生物相容性和降解率的组织工程支架。 【关键词】? 交联温度; 壳聚糖; 胶原; 京尼平; 组织工程 　　Abstract: [Objective]To investigate the changes of pore sizes,crosslinking index,swelling ration,degradation rate,cytotoxicity and biocompatibility of genipin crosslinked collagen/chitosan scaffolds affected by the different crosslinking temperature points.[Method]The freeze-dried collgaen/chitosan scaffolds crosslinked with 0.5% genipin within 24 h were divided into 3 groups by crosslinking temperature:4℃ group,20℃ group and 36℃ group.The characteristics of pore sizes,crosslinking index,swelling ratio,degradation rate,cytotoxicity and biocompatibility were evaluated.Collagen/chitosan scaffolds without crosslinking were chosen as control group.[Result]With the increase of temperature,crosslinking index was increased,but swelling ratio and degradation rate were decreased.In 4℃ group,the crosslinking index was 47.88%±6.4%,the swelling ratio was 721%±46%,and the degradation rate was decreased by 3.95%±6.4% at 4 weeks.In 20℃ group,the crosslinking index was 67.69%±3.6%,the swelling ration was 662%±72%,and the degradation rate was 0.91%±5.9% in 4 weeks.In 36℃ group,the crosslinking index was 70.32%±5.7%,the swelling ration was 635%±27%,and the degradation rate was 0.66%±7.3% at 4 weeks.The above indexes of the three groups were much better than those of control group(P0.01).[Conclusion]It was indicated that the swelling ratio and