小麦抗叶锈病近等基因系TcLr19的差异表达分析-植物病理学报.PDFVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA摇 41(4):445鄄448(2011) 藘蕛蕛蕛蕛蕛藘蕛 蕛研究简报藘 藘蕛蕛蕛蕛蕛 小麦抗叶锈病近等基因系TcLr19 的差异表达分析 1# 1,2# 1 1 1 1* 1* 陈玉婷 , 陈云芳 , 李淑凤 , 高士刚 , 闫红飞 , 杨文香 , 刘大群 1 2 ( 河北农业大学植物病理系,河北省农作物病虫害生物防治工程技术研究中心, 保定071001; 苏州出入境检验检疫局, 苏州215126) Differential expression of near鄄isogenic line TcLr19 resistant to wheat leaf rust 1 1,2 1 1 1 CHEN Yu鄄ting , CHEN Yun鄄fang , LI Shu鄄feng , GAO Shi鄄gang , YAN Hong鄄fei , YANG 1 1 1 Wen鄄xiang , LIU Da鄄qun 摇 ( Department of Plant Pathology,Agricultural University of Hebei,Biological Control Center 2 of Plant Diseases and Plant Pests of Hebei Province,Baoding 071001, Suzhou Entry鄄exit Inspection and Quarantine Bureau, Suzhou215126) Abstract: Complementary DNA amplified fragmentslength polymorphism (cDNA鄄AFLP) analysiswas em鄄 ployed to detect the differential expression of genesin TcLr19 and Thatcher inoculated with Puccinia triticina isolate05鄄21鄄116盂 (THTS). A total of 58 from 224 primer pairs displayed polymorphism between TcLr19 and Thatcher. Polymorphic transcript鄄derived fragments(TDFs) between TcLr19 and Thatcher were grouped into seven types,and five were supposed to correspond to the process of disease鄄resistant. Twelve of these TDFswere sequenced and nine were homologic with the known function genesby BLASTx analysis. Calmo鄄 dulin鄄binding protein,leucine鄄richrepeatreceptor鄄likekinaseand serine/ threoninekinaseprotein were supposed to correspond to signal transduction of plants. Stem rust resistance like protein,stress regulated protein and PDR鄄type ABC transporter9 were supposedtocorrespondtotheexpression oftheLr19 disease鄄resistant gene. Key words: wheat leaf rust