内皮脂肪酶对人脐静脉内皮细胞ICAM-1表达的影响及量效关系.docVIP

内皮脂肪酶对人脐静脉内皮细胞ICAM-1表达的影响及量效关系 方玉强，黄 岚，赵晓辉，尹扬光，康华丽，邓梦杨(第三军医大学新桥医院心内科全军心血管病研究所，400037) 提 要：目的 ndothelial lipase, EL）对人脐静脉内皮细胞（human umbilical vein endothelial cells, HUVEC）ICAM-1表达的影响及量效关系。方法 以TNF-α(10ng/ml)刺激HUVEC，RT-PCR检测附分子ICAM-1 mRNA表达的变化；以50ng/ml EL抗体进行预处理，再检测附分子mRNA表达的变化；再0~50ng/ml不同浓度EL抗体对HUVEC进行预处理，检测不同浓度EL抗体作用后ICAM－1 mRNA表达的变化。结果 HUVEC受TNF-α作用后， ICAM-1表达显著增加P0.01），该作用可为EL抗体所拮抗，这种拮抗作用呈现剂量依赖性增强(P0.01,P0.05)。结论 EL可呈剂量依赖性的促进内皮细胞表达ICAM－1，提示TNF-α作用于HUVEC产生的EL可促进附分子ICAM-1的表达。 关键词 内皮脂肪酶；HUVEC；ICAM－1；TNF-α 文献标识码： Effects of endothelial lipase on ICAM-1 expression in human umbilical vein endothelial cells FANG Yu-qiang, HUANG Lan, ZHAO Xiao-hui, YIN Yang-guang, KANG Hua-li, DENG Meng-yang (Institute of Cardiovascular Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China) Abstract: Objective Endothelial lipase (EL) modulates the monocyte adhesion to the endothelial cells. We explore the effect of EL on the expression of adhesion molecules in endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) were treated with TNF-α (10 ng/ml), TNF-α (10 ng/ml)+anti-EL antibody (0, 2.5, 5, 10, 25, 50 ng/ml, respectively) or serum-free media, then the expression levels of intercellular adhension molecule-1 (ICAM-1) mRNA were detected by RT-PCR. Results After treated with TNF-α, the ICAM-1 mRNA levels expressed by HUVECs were significantly increased. These effects were significantly antagonized by anti-EL antibody in a dose-dependent manner. Conclusion EL expressed in HUVECs stimulated by TNF-α can promote the expressions of adhesion molecules in endothelial cells, indicating EL may participate the pathophysiologic progress of coronary artery disease. Key words: endothelial lipase; HUVEC; ICAM-1; TNF-α 在冠心病动脉粥样硬化(AS)的发病过程中，内皮细胞受损、附分子表达、单核细胞等炎性细胞与血管内膜附并引起血管炎症反应是AS的关键和始动环节[1]。细胞间附分子(intercellur adhesion molocule，ICAM)能介导细胞与细胞、细胞与细胞外基质间相互附，对炎性细胞的渗出、迁移等有重要作用，因而在冠心病发病中起着重要作用。内皮脂肪酶(endothelial lipase,EL)是199