吲哚胺2,3-二氧化酶(IDO)对乙肝患者T细胞的免疫抑制作用.docVIP

吲哚胺2,3-二氧化酶（IDO）对乙肝患者T细胞的免疫抑制作用 何益平1陈永兵2 龚建平* 第一作者地址：重庆医科大学附属第二医院肝胆外科400010 第二作者地址：中国人民解放军总医院肝胆外科，北京，100853 本研究受中国博士后科学基金（20070420567）和国家自然科学基金（No.资龚建平重庆医科大学附属第二医院肝胆外科400010; [摘 要]目的 近年来，吲哚胺2,3-二氧化酶（IDO）因其参与机体的免疫调节而备受关注。本研究观察IDO在慢性乙肝病毒感染方面的免疫耐受作用，从而为重建人体主动免疫提供一种新方法。方法 采集50例慢性乙肝患者外周静脉血作为乙肝组，检测其乙肝病毒复制水平，T细胞亚群功能，以及IDOmRNA，蛋白定量以及表达活性: 2.11±0.615×103 vs 0.143±0.026×103; 蛋白定量: 0.22±0.06 vs 0.02±0.0017; 表达活性: 26.07±8.12 vs 4.98±1.65; P0.05）, IDO mRNA与HBV (r=0.502, P0.001)和ALT均正相关ALT (r=0.65, P0.01) 。另外，另外，IDOmRNA,蛋白定量和表达活性均与CD4(+) T cells (r=-0.622, -0.682, -0.549, P0.05), CD8 (+) T 细胞（r=-0.487, -367, -294, P0.05）以及CD4/CD8比值（r=-0.426, -0.533, -0.397, P0.05）负相关。结论 IDO与HBV密切相关，并且参与HBV的免疫耐受，抑制IDO的功能可以为打破乙肝病毒的免疫耐受提供一个新方法。 关键词：乙肝病毒；吲哚胺2,3-二氧化酶；免疫耐受 Immuosuppressive Effect of IDO on T cells in Patients with Chronic Hepatitis B Yi-Ping He1, Yong-Bing Chen 2, Jian-Ping Gong* The first author: Yi-Ping He, Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China The secend author: Yong-Bing Chen, Department of Hepatobiliary Surgery, Chinese PLA Genaral Hospital,Beijing,100853,China This work was supported by the grant from China Postdoctoral Science Foundation (No.20060390678 and No.20070420567) and National Natural Science Foundation Correspondence: Jian-Ping Gong, Ph.D, Department of Hepatobiliary Surgery, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China ; Abstract Object Recently indoleamine 2,3-dioxygenase (IDO) has drawn considerable attention as mechanism of immune regulation. Our study was to observe the role of IDO in immune tolerance of chronic hepatitis B (CHB), so as to provide a novel approach for reestablishment of active immunity. Method The peripheral venous blood samples were taken from 50 chronic hepatitis B (CHB) patients and HBV viral load, T lymphocyte subsets as well as the mRNA, protein and activity of IDO were detected. The correlation