川西獐牙菜对内毒素所致大鼠胆汁淤积性肝损伤的保护作用.docVIP

川西獐牙菜对内毒素所致大鼠胆汁淤积性肝损伤的保护作用 高 宇，柴 进，李绍雪，刘 畅，程 英，刘潇聪，连 伟，陈文生 （400038重庆，第三军医大学西南医院全军消化病研究所摘要目的(bile salt export pump，BSEP)和核受体 (farnesoid X-receptor，FXR)的影响。方法 20只SD雄性大鼠采用随机分为4组（每组5只）：生理盐水组、川西獐牙菜组、LPS +生理盐水组、LPS+川西獐牙菜组，所有大鼠均于处理后16h取材。统计分析各组实验大鼠肝功能指标（ALT，AST、TBIL）、胆汁流速；采用Western blot及RT-PCR观察生理盐水组、川西獐牙菜组大鼠肝组织中BSEP和FXR在转录以及蛋白水平的表达。结果 LPS+生理盐水组与生理盐水组相比肝损伤指标ALT、AST、TBIL明显升高（0.05）（0.05）（0.05）（0.05）（ 0.05）（ 0.05）（ 0.05）关键词Swertia mussotii Franch on lipopolysaccharide-induced cholestatic liver damage in rats Gao Yu, Chai Jin, Li Shaoxue, Liu Chang, Cheng Yin, Liu Xiaocong, LianWei，Chen Wensheng(Institute of Gastroenterology of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.) [Abstract] Objective To investigate the hepatic protective effect of Swertia mussotii Franch on lipopolysaccharide-induced cholestatic liver damage in rats, and the influence of Swertia mussotii Franch on the expression of hepatic bile acid transporters BSEP and nuclear?receptor FXR. Methods Twenty male SD rats were randomly divided into four groups (n=5 per group)：normal saline group，Swertia mussotii Franch group, LPS (lipopolysaccharide)+normal saline group，and LPS+Swertia mussotii Franch group. Bile flow rate was detected 16 hours after injection, and then liver samples and blood serum were collected. Hepatic biochemical indicators (ALT, AST, TBIL) were detected by biochemical analyzer. Expression of BSEP and FXR at mRNA and protein levels were detected by RT-PCR and Western blot. Results In LPS+normal saline group, the level of ALT、AST and TBil in serum were significantly higher, while bile flow rate was obviously lower than which were in normal saline group（p0.05） Swertia mussotii Franch group, the level of ALT、AST and TBil were significantly lower, while bile flow was obviously higher than which were in LPS+ normal saline group（p0.05）Swertia mussotii Franch group, the protein expression of BSEP was higher than which was in normal saline group（p0.05）FXR