甲状腺乳头状病变中端粒酶活性的检测.PDFVIP

第 38 卷　第 4 期 青 岛 大 学 医 学 院 学 报 Vol. 38 , No. 4 2002 年 12 ACTA ACADEMIAE MEDICINAE QIN GDAO UNIVERSITATIS December 　2002 甲状腺乳头状病变中端粒酶活性的检测 于建宪 　王军华 　陈 　桦 　黄维清 　卫红军 　马海燕 [摘要] 　①目的 　探讨甲状腺乳头状病变中端粒酶活性的变化及其临床意义。②方法 　采用银染端粒重复 序列扩增法 ,对 37 例病理诊断为甲状腺乳头状腺癌、乳头状腺瘤、乳头状增生的新鲜甲状腺组织进行了端粒酶活 性的定量检测。③结果 　甲状腺乳头状腺癌组织中端粒酶阳性率为68. 8 % ,乳头状腺瘤组织中为 66. 7 % ,乳头状 ( ) 增生的阳性率为 50. 0 % ,三者比较差异无显著性 P = 0. 612 ,0. 370 ,0. 410 。④结论 　端粒酶活性的定量检测对 甲状腺乳头状病变鉴别诊断意义不大。上皮乳头状增生、增生活跃的乳头状腺瘤与乳头状腺癌可能本身就是一种 疾病发展过程中的不同阶段。 [ 关键词] 　端粒酶 ; 甲状腺肿瘤;乳头状瘤 [ 中图分类号] 　R736. 1 　　[文献标识码] 　A 　　[文章编号] (2002) DETECTION OF TELOMERASE ACTIVITY IN THYROID PAPILLARY LESIONS 　Y U Jianxian , WA N G J unhua , CHEN Hua , et al 　Department of Pathology , Qingdao Municipal Hospital , Qingdao 266011 [ ABSTRACT] 　Objective 　To study the telomerase activity and its clinical significance in thyroid papillary lesions. 　Methods A modified semiquantitative polymerase chain reactionbased telomeric repeat amplification protocol assay was used to detect telomerase activi ty in 37 samples obtained at thyroidectomy , including 16 papillary cancers , 15 papillary adenomas and 6 papillary hyperplasia. 　Results 　Positive rate of telomerase was 68. 8 % for papillary carcinoma , 66. 7 % for papillary adenoma and 50. 0 % for papillary hyperplasia. The difference was not significant among the 3 groups. 　Conclusion 　A quantitative detection of telomerase activity is of no significance in dif ferential diagnosis of papillary problems. Papillary hyperplasia of epithelium , actively hyperplastic papilloadenoma and papillary adenocarci noma may be the different phases in the development of the same problem. [ KEY WORDS]