慢性非细菌性前列腺炎大鼠的尿动力学研究.docVIP

慢性非细菌性前列腺炎大鼠的尿动力学研究 张彦生 钟甘平 史庭恺 马俊林 (兰州大学第二临床医学院泌尿外科,甘肃兰州730000) 【摘　要】目的: 观察实验大鼠逐渐形成的前列腺炎和与之伴随的尿动力学改变。方法　成年Wistar雄性大鼠50只, 随机分为5个组，每组10只。除假手术组外,其他4组大鼠均在无菌条件下行去势手术，术后第二天分别连续给予稀释于花生油的苯甲酸雌二醇(E2 0.25mg/kg/d )15天及30天和苯甲酸雌二醇(E2 0.125mg/kg/d) 30天，以注射等量的花生油30天为模型对照组，假手术组仅皮下注射等量的生理盐水30天。于末次给药后第二天在麻醉下行尿动力学检查，然后做实验大鼠前列腺的组织病理学分析。结果　雌激素可诱导大鼠发生慢性非细菌性前列腺炎，E2 0.25mg/kg给药30天组排尿阈值压增加，顺应性及膀胱容量降低，与对照组及假手术组相比，差异有显著性( P 0.05)，E2 0.25mg/kg15天组和E2 0.125mg/kg30天组与对照组及假手术组相比尿动力学指标无显著性差异( P 0.05 )。结论 雌激素浓度的增加是前列腺炎逐渐产生的原因，与腺体炎症相关的尿动力学改变提示膀胱功能有改变，对其机制需做进一步研究。 关键词:前列腺炎；雌激素；尿动力学 Urodynamic changes in the rat model for chronic nonbacterial prostatic inflammation ZHANG Yan-sheng,ZHONG Gan-ping,SHI Ting-kai,MA Jun-lin(Department of Urology, Second Clinical Medicine,Lanzhou University,Lanzhou 730000, China) Abstract: Objective To observe the gradual development of prostatic inflammation and concomitant urodynamic changes of Wistar rats. Methods A total of 50 adult male Wistar rats were randomly divided into 5 groups,10 each. Except for sham operation group, 4 other groups were castrated under sterile condition. One day after operation, the rats were separately injected estradiol benzoate (0.25 mg/ kg/ day, s.c.) solved in peanut oil for 15 days and 30 days and estradiol benzoate (0.125 mg/ kg/ day, s.c.) for 30 days, the equivalent peanut oil as model control. what’s more, the sham operation group were given normal saline . After the last treatment for one day, the urodynamical recordings and histopathological analysis were done. Results Estrogen treatment can induce prostatic inflammation in the rats and different degree of inflammation and lymphocytic infiltration was found in prostate. Comparison with sham operation group and model control group, of the 30 day E2 0.25mg/kg group, voiding threshold pressure was significantly increased, while bladder capacity and compliance were significantly decreased ( P 0.05),no significant change was observed in the other groups ( P 0. 05) Conclusion E