实验方法（Experimental method）.doc

实验方法（Experimental method） 1.3 test method The aseptic operation will sterilization glass bottle samples containing 225mL 25g in sterilized saline (in the bottle I put the appropriate number of glass beads) or sterilized mortar, after sufficient shaking or grinding into homogeneous diluted solution of 1:10, 1mL Straw draw 1:10 dilution 1mL sterilization, injection containing 9mL sterile sodium brine tube, shaking tube mixing, made of diluent 1:100. Another 1mL sterilization straw, according to the operation on the line to do 10 times increments of diluted liquid, each increase diluted once, replaced with 1 1mL sterilization straw. Lactose bile salt fermentation method in 1.3.1GB/T4789.3-2003 1.3.1.1 fermentation test The sample dilution (liquid with liquid beverage and drinking water) inoculated into lactose bile fermentation tube, the front three bassoon double fermentation tube, inscribed uniform dilution 1:10 (liquid beverage and drinking water solution) 10mL, rear six, of which three for unblended fermentation tube, tube inoculation 1:10 the uniform dilution (liquid beverage and drinking water solution) 1mL, the other three tube dilution inoculation 1:100 (liquid beverage and drinking water 1:10 homogeneous diluted solution) 1mL, another 2 lactose bilesalt fermentation (or double lactose bilesalt fermentation), the samples were joined with dilute saline dilution the same as control. With good culture tube in 36 + 1 C temperature training box, 24 + 2h, such as all lactose bilesalt fermentation are acidgas, can be reported as the coliform bacteria, if the medium yellow, producing air bubbles in the window, for the production of acid and gas, then confirmed test. 1.3.1.2 confirmatory test The fermentation gas production tube were turned in eosin methylene blue agar, 36 + 1 C temperature box, cultured for 18 ~ 24h, and then removed, and observation of colony morphology, Gram staining and confirmed test. In the plate, were suspicious of coliform colonies from 1 to 2 g