展开剂的选择（Selection of unfolding agents）.docVIP

展开剂的选择（Selection of unfolding agents） Selection of unfolding agents Recently in the laboratory to detect the reaction with TLC, but not much information about the agent, our laboratory is mostly two systems: petroleum ether / ethyl acetate, chloroform / methanol, the other is little, so I resolved to make the technology more commonly used in the lab clear. Here is my search from the Internet From the experimental teaching network of Zhejiang Sci-Tech University A simple approach is based on the solubility product you choose a polar solvent, the strongest (such as alcohols, acetonitrile etc.), according to the actual situation you start slowly adding weakly polar solvent (such as carbon tetrachloride, petroleum ether and dichloromethane) polarity regulating system. To get the best expansion effect. Tell me about my secret recipe PE (60-90) EtAc/PE=1:2 EtAc/PE/AcOH=15:5:1 EtAc/AcOH/n-Butanol/H2O=2:1:1:1 In 8 years I used these four systems, there have been no question of what I have been using the ethyl acetate cyclohexane, continuously adjust the ratio, until a satisfactory Rf value, might want to add acid or alkali tailing, I three acetic acid and triethylamine. Ive been using it for years, but its ok. May as well try! Amino acids have special agent, commonly used in butanol and water, coupled with the selection of the agent to use general acid-base mixed solvent: generally have a higher solubility of developing solvent samples to polar, as samples, and then select the corresponding system. Acetic acid: petroleum ether system with greater polarity, with methanol: chloroform system, polar methanol: water: n-butanol: acetic acid system The tail can add a small amount of acetic acid or ammonia (methanol: chloroform should be good) good for I used the agent with amine: chloroform / methanol; cyclohexane / acetone / petroleum ether; ethyl acetate; methanol / water / pyridine.; launched system I used, most with benzene acetone. By adjusting the different proportion can ge