诱导痰端粒酶活性的检测与肺癌的诊治.pdfVIP

维普资讯 · 1608 · 论 著 · 诱导痰端粒酶活性的检测与肺癌的诊治 刘乾中，李秋根，彭亦平 【摘要】 目的 探讨端粒酶活性在肺癌患者诱导痰中表达的临床意义。方法 采用TRAP—PCR—ELISA法对3O 例肺癌患者诱导痰、自然痰及 15例肺部 良性病变者诱导痰中端粒酶活性进行测定。结果 肺癌患者的诱导痰、自然痰 以及非肺癌患者诱导痰中的端粒酶阳性率分别为 8O．O％、56．7％、13．3％；OD值分别为 (O．52±0．27)、 (O．35± O．25)、(O．09±0．11)。肺癌诱导痰组端粒酶的阳性率及OD值均高于 自然痰组及 良性对照组，差异有显著性意义 (P 0．01)。1O例小细胞肺癌 (SCLC)化疗后诱导痰中端粒酶OD值明显下降，与化疗前比较差异有显著性意义 (P O．O1)。结论 诱导痰中端粒酶活性的检测有助于肺癌的诊治及其与肺部 良性疾病的鉴别诊断，且优于 自然痰。 【关键词】 肺癌 ；诱导痰；端粒，末端转移酶；诊断 【中图分类号】R743．2 【文献标识码】A 【文章编号】1007—9572 (2006)19—1608—03 AssayofTelomeraseActivityinInducedSputum intheDiagnosisandTherapyofLungNeoplasms LIUQian—zhong，LI Qiu—gen，PENGYi—ping．TheRespiratoryDepartment，JiangxiChestHospital，Nanchang330006．China A【bstract】 Objective Toinvestigatetheclinicalsignificanceofdetectingtelomeraseactivityininducedsputuminpa． tientswithlungcancer．M ethods Thetelomeraseactivitiesininducedsputaandspontaneoussputafrom 30 patientswith lung cancerand15patientswithbenignlungdiseasewereassayedwithTRAP—PCR—ELISA．Reslllts Thepositiveratesoftelomer- aseactivityininducedsputum，spontaneoussputum rfomlungcancerpatients，andinducedsputum from benignlungdiseasepa— tientswererespectively8O．O％，56．7％and13．3％；andthemeanODvalueswererespectively (0．52±0．27)． (0．35± 0．25)and (0．09±O．11)．ThepositiverateandODvalueininducedsputumrfompatientswithlungcancerwasstatistically significantlyhigherthanthoseinspontaneoussputumandcontrolgroups(P O．01)．Afterchemotherapy，themeanODvalue oftelomeraseactivityininducedsputaof10casesofsmallcelllungcancer(SCLC)wasdecreasedobviously．withasignificant difference(P O．01)．Conclusion Thedetectionoftelomeraseactivityininducedsputummaybehelpfultothediangosisand therapyoflungcancer，andthedifferentialdiagnosiswithotherbenignlungdiseases；and itisbetterthanthatinspo ntaneous spu