过表达白介素-10减少脂多糖所致大鼠星形胶质细胞炎症介质的释放.pdfVIP

中南大学学报 ‘学版) JC 、 2(】1l，36(5) http：／／by．ysm．nel entSouthUnivfMedSc1 … ‘’ … … …… … 392 IL一10overexpression attenuatedexpression ofTNF-仪 and IL一113activatedbyIip0polysaccharideinastrocytes HEZhenghua，XIAOMuzhang，GUOQulian (1．DepartmentofAnesthesiology；2．DepartmentofCosmeticandPlasticSurger)， XiangyaHospital，CentralSouthUniversity，Changsha410008，China) Abstract： Objective Toinvestigatetheinterventioneffectoflentivirusexpressinghuman IL一10fLV—hIL．101onactivatedastroeytes．M ethods DITNC1celllinewastreatedwithdifferent concentrationsoflip0p0lysaccharide(LPS)andtimepoints．Theexpressionofproinflammatorycyto— kinestumornecrosisfactor— (TNF一仅)andinterleukinb．1B(IL一1B)wasdetectedbyRT。PCRand ELISA．Moreover，theeffectontheexpressionofproinflammatoryeytokinesTNF—OLandIL．1Bwas analyzedinDITNClcelllinesinfectedwithandwithoutLV—hlL．10．Results Theexpressionof TNF—Or．andIL一1BwasincreasedinDITNC1inducedbyLPS．TheexpressionofIL一10wasupregu— latedinDITNC1infectedwith LV—hIL．10．TNF— andIL一1BwereinhibitedbyIL一10 overexpres— sioninDITNC1activedbyLPS．Conclusion DITNC1iSactivatedbyLPSandsecretesproinflam— matorycytokinesTNF．仅andIL一1Basimmune1ikecells，andtheseactivationiSinhibitedbyhlL—l0 overexpression． Keywords： lentivirusvector； IL一10； astrocyte； proinflammatorycytokines 过表达 白介素．10减少脂多糖所致 大鼠星形胶质细胞炎症介质的释放 贺正华’，肖目张 ，郭曲练 (中南大学湘雅医院 1．麻醉科；2．美容整形外科，长沙410008) [摘要] 目的：观察携带人IL—l0基因的慢病毒(LV—hlL一10)对激活的星形胶质细胞的干预作用 方法 ： 确定脂多糖 (LPS)诱导DITNC1细胞的最佳浓度和时间，利用表达 白介素一1O(IL一10)的慢病毒感染DITNCI 细胞，使用 liT-PCR和ELISA法检测LPS诱导下DITNC1细胞促炎因子TNF—OL，IL一1p的表达改变。结果：L