低盐度胁迫对银鲳幼鱼肾脏和肌肉抗氧化酶活力的影响.doc

Effects of Low Salinity on Antioxidant Enzymes Activities in kidney and muscle of Juvenile Silver Pomfret Pampus argenteus Yin Fei, Peng Shiming, Sun Peng, Shi Zhaohong* Key and Open Laboratory of Marine and Estuarine Fisheries，Ministry of Agriculture of China，East China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Shanghai 200090，China ABSTRACT Silver pomfret (Pampus argenteus) is an important fish species in China, whose fatality is strongly affected by marine salinity. To better understand the tolerance of sliver pomfret to varying environmental salinity at its early developmental stages, the influence of salinity on the activities of five antioxidant enzymes — superoxide Dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST), and glutathione reductase (GR) — in the kidney and muscle of juvenile silver pomfret was investigated in this study. Divided group A, B, C, D at random, the juvenile silver pomfrets were then treated with different salinity levels of 25, 20, 15, and 10 with time periods of 24, 48, 96, and 120 hours respectively. Generally speaking, the results show that the renal and muscular SOD as well as the renal CAT activities first rise then drop (P0.05), while the muscular CAT activity increases with the elapse of treatment time (P0.05) and peaked at 96 h, then decreased at 120 h, while the muscular CAT activity in group D increased consistently and peaked at 120h. The renal GPX activity decreases significantly (P0.05) as the salinity drops and treatment time prolongs with its lowest level occurring at 96h in group D, while the muscular GPX activity increases first then decrease (P0.05); moreover the renal and muscular GPX activities vary reversely in compensation. On a whole, the renal and muscular GST and GR activities first rise then drop (P0.05) with the drop of salinity and elapse of time. To sum up, the results acquired from study indicate that the salinity decrease could effective